Dictionary » S » Scrape

Scrape

scrape

1. To rub over the surface of (something) with a sharp or rough instrument; to rub over with something that roughens by removing portions of the surface; to grate harshly over; to abrade; to make even, or bring to a required condition or form, by moving the sharp edge of an instrument breadthwise over the surface with pressure, cutting away excesses and superfluous parts; to make smooth or clean; as, to scrape a bone with a knife; to scrape a metal plate to an even surface.

2. To remove by rubbing or scraping (in the sense above). I will also scrape her dust from her, and make her like the top of a rock. (Ezek. Xxvi. 4)

3. To collect by, or as by, a process of scraping; to gather in small portions by laborius effort; hence, to acquire avariciously and save penuriously; often followed by together or up; as, to scrape money together. The prelatical party complained that, to swell a number the nonconformists did not choose, but scrape, subscribers. (Fuller)

4. To express disapprobation of, as a play, or to silence, as a speaker, by drawing the feet back and forth upon the floor; usually with down. To scrape acquaintance, to seek acquaintance otherwise than by an introduction. He tried to scrape acquaintance with her, but failed ignominiously. (G. W. Cable)

Origin: Icel. Skrapa; akin to Sw. Skrapa, Dan. Skrabe, D. Schrapen, schrabben, G. Schrappen, and prob. To E. Sharp.


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Alizarin Red Staining on scaffolds...

... being done on a plate/well. For quantification purpose, we use the de-staining solution and take results using a plate reader. Is it necessary to scrape cells? I cannot scrape cells from a scaffold. Thank you P.S. : I'm new to tissue eningeering..

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by cshmech
Fri Jan 11, 2013 10:13 pm
 
Forum: Cell Biology
Topic: Alizarin Red Staining on scaffolds...
Replies: 1
Views: 1329

Re:

this is just poor kitchne hygiene. If you call letting your pots sit over night in soap and water poor kitchen hygiene, then you are showing me that soap could also be a variable to configuring its growth when I go to the lab. Yet it's valid that poor kitchen hygiene was at play due to my concern t...

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by WillyTNo
Tue Jun 05, 2012 7:03 pm
 
Forum: Microbiology
Topic: Growth on metal pot?!
Replies: 24
Views: 10175

Re: thin layer chromatography

If you've easy access to a UV-Vis spec, you could scrape off the bands, elute them and take some spectra -- then assign the kids into the literature to find likely candidates. It could get complicated if some pigments are co-migrating, but it would ...

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by jonmoulton
Mon Nov 28, 2011 10:45 pm
 
Forum: Molecular Biology
Topic: thin layer chromatography
Replies: 4
Views: 2203

Question about "Skin"

... the outer most skin layer. We slough off some cells as well as bacteria and fungi that are hanging out on our skin. If we scratch hard enough or scrape of against something we can get an abrasion or a laceration where the scratch goes down to the dermis and hyperdermis where we have capillaries ...

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by daniel.kurz
Mon Nov 21, 2011 6:58 am
 
Forum: Cell Biology
Topic: Question about "Skin"
Replies: 2
Views: 1429

Re: Western Blot TroubleShooting

I harvest my samples using RIPA buffer + protease inhibitor on ice. I use a 6 well dish and I scrape the samples off on ice. I rotate the samples at 4C for 1 hour for lysis. I then centrifuge for 30 min. at 13,000 rpm at 4C. I take off the supernatant. I add 2X SDS PAGE ...

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by mutant11
Mon Nov 21, 2011 3:11 am
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3355
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