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Saturation analysis

saturation analysis --> competitive binding assay

general term for an assay in which a binder competes for labelled versus unlabelled ligand; following separation of free and bound ligand, the ligand (the analyte assayed) is quantitated by relating bound and unbound ratios to known standards.

See: enzyme-linked immunosorbent assay, radioreceptor assay, immunoassay, enzyme-multiplied immunoassay technique, radioimmunoassay.

Synonym: displacement analysis, saturation analysis.

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Cell Membrane

... diffusion along a concentration gradient <<<< This one? d. Saturation e. Fick’s Law. Also, is Extracellular fluid? a) dilute salt soltn surrounding cell b)another name for interstitial fluid Analysis of the cells in the intestine reveal that they are permeable to water, ...

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by Nicolodn
Sat Sep 18, 2010 8:05 am
Forum: Cell Biology
Topic: Cell Membrane
Replies: 1
Views: 6015

ELISA hormon assay

... are used to linearize the curve over the range of at least 10-90% saturation. Here is a brief overview of ELISA from Millipore Corp. http://www.chemicon.com/resource/ANT101/a2C.asp ... paper (in the public domain from PubMed) comparing different models for analysis of standard curves http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=270131&blobtype=pdf ...

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by blcr11
Sat Mar 31, 2007 5:00 pm
Forum: General Discussion
Topic: ELISA hormon assay
Replies: 4
Views: 3778

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