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Saturate

saturate

Filled to repletion; saturated; soaked. Dries his feathers saturate with dew. (Cowper) The sand beneath our feet is saturate With blood of martyrs. (Longfellow)

Origin: L. Saturatus, p. P.

1. To cause to become completely penetrated, impregnated, or soaked; to fill fully; to sate. Innumerable flocks and herbs covered that vast expanse of emerald meadow saturated with the moisture of the atlantic. (Macaulay) Fill and saturate each kind With good according to its mind. (Emerson)

2. (Science: chemistry) To satisfy the affinity of; to cause to become inert by chemical combination with all that it can hold; as, to saturate phosphorus with chlorine.

Origin: L. Saturatus, p.p. Of saturate to saturate, fr. Satur full of food, sated. See Satire.


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Re: Removing MBP tag

... the column with digestion and you will have your protein in the flowthrought, be carefull in pass the sample at low flow and be sure of don´t saturate the column. collect fractions and analize, if you have contamination gel filtration is an alternative but it has sample volume limitations. ...

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by protold
Thu Aug 11, 2011 6:07 am
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 6317

Dissociation Constants

not really, it's similar as Michaelis with enzymes, so you won't get twice so much bound ligands ;) Usually, 10× more is taken to fully saturate ;)

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by JackBean
Sun May 16, 2010 8:00 pm
 
Forum: Molecular Biology
Topic: Dissociation Constants
Replies: 1
Views: 836

Dissociation Constants

... constant (Kd) of 50nM for instance. That means that the receptor is half bound by ligand when the concentration of the ligand is 50nM. To fully saturate a receptor, would one use twice as much ligand? 100nM in this case? Thanks for your help with this.... All the best :)

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by pmngrad
Sun May 16, 2010 7:06 pm
 
Forum: Molecular Biology
Topic: Dissociation Constants
Replies: 1
Views: 836

Re: Colour of veins

... and the deoxygenated blood is dark red. Even if some diffusion happen when venous blood get in contact with air this diffusion isn't enough to saturate all of the hemoglobin.

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by Swede
Sun Feb 21, 2010 9:36 pm
 
Forum: Human Biology
Topic: Colour of veins
Replies: 5
Views: 4771

Biology essay on red blood cells

... hemoglobin was measured and then separated to determine how much of the hemoglobin had all of its binding sites filled with oxygen. The amount of saturate hemoglobin was compared with the total amount of hemoglobin to yield the percentage of hemoglobin saturation. Some results of the study are ...

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by addicted
Thu Apr 17, 2008 10:31 pm
 
Forum: Human Biology
Topic: Biology essay on red blood cells
Replies: 5
Views: 3347
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