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Samples

sample size

The number of units (persons, animals, patients, specified circumstances, etc.) in a population to be studied. The sample size should be big enough to have a high likelihood of detecting a true difference between two groups.


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amplification in no RT control

... I am getting amplification in all of my no RT controls (4 treatments and an untreated), although this is occurring ~20 cycles later than RT+ samples. I am getting single peak melt curves and my NTC is not amplifying. This suggests to me that I am not getting dimers and my reagents are 'good' ...

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by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 1
Views: 47

An Overview of Embryonic Stem Cells

... Adult Stem Cells are there to repair any damage that has been done to tissue and rebuild it back to its original state. The company offers many samples from different organs and systems of mice and rats. Its Tumor Stem Cells samples are extremely valuable to those who need to compare them to ...

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by 2444256703
Thu Jan 29, 2015 6:48 am
 
Forum: Cell Biology
Topic: An Overview of Embryonic Stem Cells
Replies: 1
Views: 579

How would I do this experiment? [Advanced]

As you must be able to grow the bacteria (otherwise you cannot perform the test at all), it would be better to perform dilution of your samples and spread aliquots of such on plates with growth agar (for E.coli simple LB, for Kefir, no idea). Let them growth (E.coli for 1 day at 37°C) and ...

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by JackBean
Fri Dec 19, 2014 2:46 pm
 
Forum: Cell Biology
Topic: How would I do this experiment? [Advanced]
Replies: 2
Views: 1034

separation problem with GelRed

... band separation: 1. Reduce the amount of loaded DNA. Overloading of DNA often results in band smearing. The recommended amount for ladders and samples of known concentration is 50-200 ng/lane. If the concentration is unknown, try loading one half or one third of the usual amount of DNA. 2. ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19576

Oxygen requirements of eukaryotes

you do it one by one, not all together of course. And it's not that difficult to do that with say 200 samples. Just curious about the results you got.

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by JackBean
Sat Nov 08, 2014 5:13 pm
 
Forum: Microbiology
Topic: Oxygen requirements of eukaryotes
Replies: 7
Views: 4481
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