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Safranin o

safranin O

A mixture of dimethyl-and trimethylphenosafranin chloride, a basic red dye that exhibits orange metachromasia; used in histology as a nuclear stain, in microbiology as a counterstain in the gram method, and to demonstrate enterochromaffin.


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Re: Tips for staining (light microscopy)

... wastewater treatment, the time standards are different. Wastewater Gram stains typically stain Crystal Violet and Gram's Iodine for 2 minutes, and Safranin counter-stain for 30 seconds. 3) I have no input on this subject, except to say that the stain should stick to the organism, and that excess ...

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by PsycoCarrot
Fri Jul 06, 2012 11:03 pm
 
Forum: Microbiology
Topic: Tips for staining (light microscopy)
Replies: 3
Views: 4078

Staining slides with Food dye? (Or any household item)

/:quote If you need to Gram Stain, I think you really need to use Crystal Violet counterstained with either Safranin or Fuschin, quote:/: do that that's more easier. and it works well

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by jwalin
Fri Jan 22, 2010 12:32 pm
 
Forum: Microbiology
Topic: Staining slides with Food dye? (Or any household item)
Replies: 7
Views: 8117

Re: Staining slides with Food dye? (Or any household item)

... for non-differential staining of bacteria. If you need to Gram Stain, I think you really need to use Crystal Violet counterstained with either Safranin or Fuschin, although I've never had to scrounge for materials, so I could be wrong. I can't think of any commonly available preparations of ...

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by billw
Fri Jan 22, 2010 12:51 am
 
Forum: Microbiology
Topic: Staining slides with Food dye? (Or any household item)
Replies: 7
Views: 8117

Definition of Primary Stain?

... will actually stain what you want to see. in the case of the Gram stain, that is the particular structure of the cell wall of the Gram+ bacteria. Safranin is a non specific stain that will just allow the visualization of the bits that have not already been stained. Gram+ bacteria do also take ...

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by canalon
Fri Oct 02, 2009 6:28 pm
 
Forum: Microbiology
Topic: Definition of Primary Stain?
Replies: 1
Views: 17272

Definition of Primary Stain?

... Staining; 1. Apply Primary Stain (Crystal violet) 2. Add Trapping agent (Gram's Iodine.) 3. Decolorize (With alcohol or acetone) 4. Counterstain (Safranin or basic Fuchsin) Anyone able to assist? Thanks in advance :)

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by Aric
Fri Oct 02, 2009 5:27 pm
 
Forum: Microbiology
Topic: Definition of Primary Stain?
Replies: 1
Views: 17272
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