SSCP

... reaction from each egg. (I had a look at your single-tube protocol. Have you tried this yet?). Maybe you could type the mtDNA (sequencing, RFLP, SSCP) to get maternal information, and find a variable region of the male sex chromosome (that has no corresponding region on the female sex chromosome ...

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... of PCR (3) Important Publications at Cell Important Publications at Nature Important Publications at Science Variants of PCR (2) PCR Manual PCR SSCP PCR RFLP Variants of PCR (1) AFLP PCR Alu-PCR Asymmetric PCR Colony PCR Protocols Competitive and/or Quantitative RT-PCR Degenerate PCR In Situ ...

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I found it's very difficult to do PCR Elisa and PCR-SSCP. Here are some protocols of PCR variants I found. http://pcrworld.blogspot.com/ The PCR-SSCP has been using to probe point mutation, but there are not a lot of publications I can refer. I wonder ...

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SSCP (Single-Strand Conformation Polymorphism try it, it may work for you

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... strand of the fragment. This is commonly done to, for example, incorporate a label into the amplified strand for detection (as in one method of SSCP).

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