Rupture

... It can be caused by holding breath while ascending. Basically if you hold your breath and then ascend the air inside you lungs expands and can rupture them.

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... If the bacteria were in a HYPERtonic solution, the plasma membrane would weaken and/or collapse and be “paralyzed” (plasmolysis). To prevent rupture of the cell membrane, the bacteria would have to be in an isotonic solution. Gram-negative bacteria are called SPHEROPLASTS and DO NOT completely ...

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... are also froze using this technique in the sperm bank. When cells freeze under normal condition, the frozen water inside the cells will cause the rupture of cells. My doubt is, how can cryonic freezing helps to prevent the sperms from rupturing?

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... shell, just the part above the embryo, but it seems like removing the shell is at least possible. It's very difficult to remove the shell and not rupture the membrane underneath it, though.

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... of cell walls damaged by generation of competent cells and the electroporation process, maintain isotonicity to prevent cell death by osmotic rupture and SOC contains glucose for easy energy conversion. (R. Hopper 1995 Genetic Engineering News 15 (9) 18.)

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