Room temperature

... (where it is located) is being altered in the cardiac tissue when the temperature is raised and lowered (+ - 10 degree) [by external factors] like the room temperature is 20degree and the temp is raised to 30 degree and temp is loweres to 10 ...

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um.. if normal for the temperature 20 degree as room temp , it raise to 30 degree , and lower to 10 degree temperature change caused by external factor abt ...

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... a big role in how long it will last. DNA that left inside and dry at room temperature can last for years, such as that found on hairs in a hair brush. DNA in harsh conditions ...

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Somewhat better than room temperature...

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... enzymes and make sticky(cohesive) end ligation. I used Quick ligase at room temperature(25 degrees) tried 5 minutes as the protocol said (NEB)alternatively try 25 minutes ...

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