Dictionary » R » Rna splicing

Rna splicing

A two step reaction in which introns are removed from a primary rna transcript and exons are joined together to form a mature mrna. An intron usually contains a clear signal for splicing, but in some cases a splicing signal may be masked by a regulatory protein. This results in alternative splicing. In rare cases a pre-mrna may contain several ambiguous splicing signals, resulting in a few alternatively spliced mrna.


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Re: qPCR vs Northern Blot

... - the Northern signal was maintained, the PRC signal decreased. A splicing change could still produce that result, as could the RNA degradation Jack mentioned.

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by jonmoulton
Mon Nov 11, 2013 4:38 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2784

Re: qPCR vs Northern Blot

I assume you are assaying aliquots of the same RNA samples by the two methods. Does your Northern probe bind between the binding sites of your PCR primers? If not, it is conceivable that a change in splicing in the presence of the shRNA causes your Northern target to be spliced out ...

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by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2784

Use of cDNA when studying splicing

Hi I've read a few studies where the authors aim to study the association of certain proteins to mRNA in a splicing-dependent manner. Mainly they compared samples containing spliced mRNA with cDNA-samples, in a way that to me seemed as if they insinuated that the cDNA samples ...

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by nukeman
Wed May 15, 2013 11:33 am
 
Forum: Molecular Biology
Topic: Use of cDNA when studying splicing
Replies: 1
Views: 837

Re: Muscular dystrophy inheritance question

... downstream of the AAAAAAA the sequence is spliced to different sequences depending on the allele? Why, though, would the male show alternative splicing if he is homozygous? I've clearly not worked out this problem correctly. As you learn more about this one, please post it here.

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by jonmoulton
Thu Jan 10, 2013 3:59 pm
 
Forum: Molecular Biology
Topic: Muscular dystrophy inheritance question
Replies: 7
Views: 5684

Re:

... result (despite the exact same procedure and minimal scope for human error). My supervisor suggested that this could be something to do with the splicing nature of the gene.... he suggested that we stop trying to clone the whole gene and look at the splicing nature of ligase 6 before we re-attempt ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3386
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