Rna splicing

... the open reading frames, so we pretty much know where most of the RNAs come from (we think - but see Jack's post). The trick is to assign function ... will be keeping folks busy for many years. Then there is alternative splicing, which can produce several different protein structures from the ...

See entire post

... insertion, deletion and substitution are possible consequences of RNA splicing.

See entire post

Ques1: Phosphorylation of RNA pol which are the roles of the phosphorylaton of C terminal Domain of RNA polymerase? ... 3? not too sure which else... Ques 2: Which may not be consequences of RNA splicing 1. Insertion of amino acids in the protein sequence 2. Deletion of amino acids ...

See entire post

... can be of effect at any stage. Because of faulty promotor, the gene may not be transcribed. There may be something wrong with the mRNA (missing splicing points, wrong iniciation/polyA signal, whatever) leading to significantly reduced half-life of the mRNA. There may be STOP codon in middle ...

See entire post

... assemble to a spliceosome. My initial thought was to use in vitro splicing assay with a couple of extra steps. 1. Ligate a traget gene to a vector with some kind of bacteriophage RNA-pol promotor 2. In vitro transcription with radioactive nucleotides 3. Extraction ...

See entire post