Dictionary » R » Rna splicing

Rna splicing

A two step reaction in which introns are removed from a primary rna transcript and exons are joined together to form a mature mrna. An intron usually contains a clear signal for splicing, but in some cases a splicing signal may be masked by a regulatory protein. This results in alternative splicing. In rare cases a pre-mrna may contain several ambiguous splicing signals, resulting in a few alternatively spliced mrna.


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Re: qPCR vs Northern Blot

... - the Northern signal was maintained, the PRC signal decreased. A splicing change could still produce that result, as could the RNA degradation Jack mentioned.

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by jonmoulton
Mon Nov 11, 2013 4:38 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1270

Re: qPCR vs Northern Blot

I assume you are assaying aliquots of the same RNA samples by the two methods. Does your Northern probe bind between the binding sites of your PCR primers? If not, it is conceivable that a change in splicing in the presence of the shRNA causes your Northern target to be spliced out ...

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by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1270

Re: Have all human genes been identified?

... the open reading frames, so we pretty much know where most of the RNAs come from (we think - but see Jack's post). The trick is to assign function ... will be keeping folks busy for many years. Then there is alternative splicing, which can produce several different protein structures from the ...

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by jonmoulton
Thu Nov 29, 2012 6:43 pm
 
Forum: Molecular Biology
Topic: Have all human genes been identified?
Replies: 3
Views: 1194

RNA

... insertion, deletion and substitution are possible consequences of RNA splicing.

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by revision
Mon Nov 26, 2012 12:01 pm
 
Forum: Molecular Biology
Topic: RNA
Replies: 6
Views: 7206

RNA

Ques1: Phosphorylation of RNA pol which are the roles of the phosphorylaton of C terminal Domain of RNA polymerase? ... 3? not too sure which else... Ques 2: Which may not be consequences of RNA splicing 1. Insertion of amino acids in the protein sequence 2. Deletion of amino acids ...

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by 2468
Tue Nov 20, 2012 6:03 pm
 
Forum: Molecular Biology
Topic: RNA
Replies: 6
Views: 7206
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