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Rna polymerases

RNA polymerase

(Science: enzyme molecular biology) An enzyme that polymerise ribonucleotides in accordance with the information present in dNA.

prokaryotes have a single enzyme for the three rNA types that is subject to stringent regulatory mechanisms. Eukaryotes have type I that synthesises all rRNA except the 5S component, type II that synthesises mRNA and hnRNA and type III that synthesises tRNA and the 5S component of rRNA.


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Re: Isolation during transcription

... higher the yield of polymerase If all you want to do is isolate some RNA polymerase, there are far easier ways to do it (and which don't involve viruses). In fact, some of these polymerases are sold commercially. then you need a technique to synthesis telomerase ...

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by AstraSequi
Fri May 04, 2012 7:12 pm
 
Forum: Molecular Biology
Topic: Isolation during transcription
Replies: 2
Views: 999

T7 Expression System

... to introduce a heat-inducible plasmid containing the gene for the T7 RNA polymerase under the control of an E.Coli promoter into the cells, and ... of labelled amino acids, and Rifampicin to inhibit endogenous RNA polymerases in the hope of translating the gene of interest, labelled, and ...

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by TjCooper
Sat Dec 31, 2011 5:46 pm
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2121

the RNA synthesized on one of the DNA strands is ..........?

Yes, it is all of them. All RNAs are synthesized by RNA polymerases, and all RNA polymerases use one strand of DNA as the template strand to synthesize single-stranded ...

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by aptitude
Tue Nov 08, 2011 6:38 am
 
Forum: Cell Biology
Topic: the RNA synthesized on one of the DNA strands is ..........?
Replies: 4
Views: 1575

Re: Theories - Origin of Life

... told you already dozen times, the crucial part of the ribosome are the rRNAs, the proteins are just added cosmetics. Ribosomes are made from approx ... a cell's genetic information. During transcription, Enzymes called RNA polymerases build RNA molecules that are complementary to a portion of one ...

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by scottie
Tue Oct 18, 2011 7:40 pm
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 548
Views: 535444

microbiology-archeobacteria and eubacteria

... two microorganisms. Both bacteria and archaea have different Ribosomal RNAs (rRNA). Archea have three RNA polymerases like eukaryotes, but bacteria have only one. Archaea have cell walls that lack ...

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by bellyjelly
Tue Jul 12, 2011 8:08 am
 
Forum: Microbiology
Topic: microbiology-archeobacteria and eubacteria
Replies: 11
Views: 14311
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