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Rna polymerase

RNA polymerase

(Science: enzyme molecular biology) An enzyme that polymerise ribonucleotides in accordance with the information present in dNA.

prokaryotes have a single enzyme for the three rNA types that is subject to stringent regulatory mechanisms. Eukaryotes have type I that synthesises all rRNA except the 5S component, type II that synthesises mRNA and hnRNA and type III that synthesises tRNA and the 5S component of rRNA. An enzyme that contributes to the formation of RNA from DNA templates.


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Taqman probe design using FISH probe (need help)

... temperature such that it will remain hybridized at PCR extension temperature (but not so high as a fish probe) and will be hydrolysed by Taq DNA polymerase at every replication, thus releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 586

Re: Purpose of a primase/polymerase localized to the cytosol?

Hi, I have some naïve thoughts about this situation: If it is an RNA pol, it may be helping maintain/regulate RNA levels in cytosol by e.g. recharging decaying RNA levels in cytosols for extended translation. If it is a DNA pol, it might be a long surviving ...

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by Hrushikesh
Fri Dec 12, 2014 8:40 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1612

Purpose of a primase/polymerase localized to the cytosol?

Hi, I recently read a paper, "PrimPol, an Archaic Primase/Polymerase Operating in Human Cells" by Garcia-Gomez et al. This polymerase was largely localized to the cytosol - I'm wondering if anyone has ideas as to why that would be the case or ...

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by rhardison
Fri Oct 03, 2014 8:51 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1612

Re: Any ideas for vaccine development or anti-viral drugs???

... effectively interfere with the replication of several positive-strand RNA viruses in cell culture. The filoviruses, Marburg virus and Ebola virus ... of viral mRNAs for the viral proteins (VPs) VP24, VP35, and RNA polymerase L protected rodents in both pre- and post-exposure therapeutic ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12749

Annealing Temparature

... at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 2616
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