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Reverse transcription



The process of making a double stranded DNA molecule from a single stranded RNA template through the enzyme, reverse transcriptase.


It is called reverse transcription because it is a process in opposite or reverse of transcription.

See also: reverse transcriptase.

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... but I don't see reason why should you amplify only short pieces during reverse transcription.

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by JackBean
Mon Jan 28, 2013 1:49 pm
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 5610


... curve-the standard. I should quantitate the transcript (RNA) before reverse transcribe them into cDNA. I wonder I should serially dilute the transcript and perform reverse transcription? Or I should synthesis the cDNA first and only perform serial dilution ...

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by xiaolong88
Tue Dec 11, 2012 4:58 pm
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 2137

RT PCR contamination.

I am running a reverse transcription PCR (cDNA synthesis) and am getting contamination. Currently I run two negative controls, a -RT ...

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by georgewiggins
Thu Sep 06, 2012 9:49 pm
Forum: Molecular Biology
Topic: RT PCR contamination.
Replies: 2
Views: 4161

PCR from cDNA question

... to eliminate hairpin structures. The cDNA was obtained using iScript reverse-transcriptase, primed by random hexamers. Curiously, the same cDNA ... specific gene products: for some proteases, very bright bands. for some transcription factors highly expressed at the same time, no bands at all. ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 3230

Real Time reverse transcription PCR Questions

... my understanding of Real Time PCR from a mRNA sample (Real Time reverse transcription PCR). The protocol I have in mind is to transfect my cells with my desired proteins ...

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by wessH
Sun Jun 03, 2012 2:21 pm
Forum: Molecular Biology
Topic: Real Time reverse transcription PCR Questions
Replies: 0
Views: 2490
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