Reverse transcription

... but I don't see reason why should you amplify only short pieces during reverse transcription.

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... curve-the standard. I should quantitate the transcript (RNA) before reverse transcribe them into cDNA. I wonder I should serially dilute the transcript and perform reverse transcription? Or I should synthesis the cDNA first and only perform serial dilution ...

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I am running a reverse transcription PCR (cDNA synthesis) and am getting contamination. Currently I run two negative controls, a -RT ...

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... to eliminate hairpin structures. The cDNA was obtained using iScript reverse-transcriptase, primed by random hexamers. Curiously, the same cDNA ... specific gene products: for some proteases, very bright bands. for some transcription factors highly expressed at the same time, no bands at all. ...

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... my understanding of Real Time PCR from a mRNA sample (Real Time reverse transcription PCR). The protocol I have in mind is to transfect my cells with my desired proteins ...

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