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Reverse transcriptase



A DNA polymerase enzyme that catalyzes the process of reverse transcription.


Reverse transcriptase makes use of RNA molecule as a template for the synthesis of a complementary DNA strand. It is produced by HIV and other retroviruses in order for them to synthesize DNA from their viral RNA.

Reverse transcription is also found to be involved in the movement of certain mobile genetic elements, such as the Ty plasmid in yeast, in the replication of other viruses such as hepatitis B and possibly in the generation of mammalian pseudogenes.

It was discovered in 1970 by two independent scientists, Howard Temin and David Baltimore.

Synonym: RNA-dependent DNA polymerase, transcriptase.
See also: reverse transcription.

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targeting the Aids virus

... mutants out there. We must somehow use an irreversible inhibitor on the reverse transcriptase enzyme.

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by Sumanth001
Fri Sep 27, 2013 12:40 pm
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Topic: targeting the Aids virus
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Views: 2052

overexpression xylanase gene

... PCR. i didnt know either he's talking about real time PCR or reverse transcriptase PCR. what do you think about the clues? how can i relate it to what i have to ...

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by nekonara
Fri Dec 14, 2012 7:50 am
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Topic: overexpression xylanase gene
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... to eliminate hairpin structures. The cDNA was obtained using iScript reverse-transcriptase, primed by random hexamers. Curiously, the same cDNA gives weird results when ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
Forum: Molecular Biology
Topic: PCR from cDNA question
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delete cDNA stop codon for fusion with fluorescent tag

Hi, Can I use the normal reverse transcriptase kit to obtain the cDNA instead of those cDNA construction kit which contains a specialized vector? ...

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by arcas
Fri Apr 13, 2012 10:10 am
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Topic: delete cDNA stop codon for fusion with fluorescent tag
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Viral budding

... mention the reproduction process of retroviruses with the assistance of reverse transcriptase. Taking the HIV virus as an example, upon completion of viral proteins being ...

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by Poggie
Wed Nov 30, 2011 7:36 pm
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Topic: Viral budding
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