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Restriction site


noun, plural: restriction sites

The site in a polynucleotide chain as to where the restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.


The restriction enzyme recognizes the site as to where it cuts via a specific sequence of nucleotides along the polynucleotide chain. For example, when the restriction enzyme, Eco RI, recognizes the palindromic sequence GAATTC, it cuts between G and A on the top and bottom strands.


See also: restriction enzyme

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Re: question for evolutionary biologist.

... Mycoplama capricolum subspecies capricolum recipient cells in which the restriction modification system had been inactivated. This synthetic M. mycoide ... system” was inactivated. If you listen to the video on Venter's site you will get a good idea as to what they actually did. As to your comment ...

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by scottie
Thu Sep 13, 2012 1:22 pm
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 34557

isolation of plasmid (Midi prep)

... band. PCR verify presence of insert in vector. 2-something wrong with restriction reaction (verify if your enzymes work on different sample) 3-there is a methylation of restriction site that blocks it for restriction enzyme. (unlikely but possible) 4-altered mobility ...

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by triton
Mon Aug 27, 2012 4:46 pm
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 6112

Restrction digest on a plasmid--how many fragments?

You are right. In a multiple cloning site, the restriction site are usually within less than20 to 30 bp of one another. Look at your plasmid map. And ...

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by canalon
Sat Jun 16, 2012 12:57 am
Forum: General Discussion
Topic: Restrction digest on a plasmid--how many fragments?
Replies: 1
Views: 2201

Real Time reverse transcription PCR Questions

... is the same orientation as DNA right? For example, lets say the NCBI website gives me the following sequence (please disregard if they are good primers ... one is correct and why? I think I understand the concept behind adding restriction sites. They are added to the 5' end and you need to add ...

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by wessH
Sun Jun 03, 2012 2:21 pm
Forum: Molecular Biology
Topic: Real Time reverse transcription PCR Questions
Replies: 0
Views: 2939

ligation problem although try everything,suggestions?

You don't say what you do after restriction. Do you remove restriction enzymes? Or simply kill restrictazes by heat-shocking? ... this way. If you don't have 2-3 nucleotides added to your restriction site in primers, it could lead to problems with inefficient digestion as well. These ...

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by dustman
Thu May 24, 2012 10:42 am
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 5457
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