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Dictionary » R » Restriction site

Restriction site

Definition

noun, plural: restriction sites

The site in a polynucleotide chain as to where the restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.


Supplement

The restriction enzyme recognizes the site as to where it cuts via a specific sequence of nucleotides along the polynucleotide chain. For example, when the restriction enzyme, Eco RI, recognizes the palindromic sequence GAATTC, it cuts between G and A on the top and bottom strands.


Synonyms:


See also: restriction enzyme

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Uses for the PCR technique?

another very elegant and extremely widely used scientific application is site-directed mutagenesis, commonly referred to as quickchange pcr, probably ... the template DNA must be eliminated by enzymatic digestion with a restriction enzyme specific for methylated DNA. The template, which for this ...

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by MrMistery
Mon May 04, 2009 11:41 pm
 
Forum: Genetics
Topic: Uses for the PCR technique?
Replies: 4
Views: 1343

constuction of a full length gene

... but you could just PCR them up with ends that can be cut by different restriction enzymes, so that the site on the end of one partial copy matches the site at the beginning of the next one. Then ...

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by MrMistery
Thu Apr 30, 2009 12:04 am
 
Forum: Molecular Biology
Topic: constuction of a full length gene
Replies: 1
Views: 236

Restriction fragment length polymorphisms

RFLPs are no regions. It stands for Restriction Fragment Length Polymorphism. In short you PCR amplify a region wher you ... then digest the PCR product. The mutation will change the restriction site and change the length of the product, so the profile will change.

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by canalon
Wed Apr 22, 2009 2:13 pm
 
Forum: Molecular Biology
Topic: Restriction fragment length polymorphisms
Replies: 1
Views: 252

DNA GEL ELECTROPHORESIS

... of the band. And different restiction enzyme have different cutting site, so likely different fragment size: think of divinding a book in fragments ... expect the different fragments to be the same size. It is the same with restriction sites.

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by canalon
Wed Mar 18, 2009 1:23 am
 
Forum: Molecular Biology
Topic: DNA GEL ELECTROPHORESIS
Replies: 5
Views: 1017

Re: Question regarding Restriction Enzymes

... and would potentially re-ligate anyway. Rather, there might not be one restriction site bordering the sequence you want to clone and the identical restriction site in the plasmid. ...

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by jonmoulton
Tue Feb 17, 2009 11:57 pm
 
Forum: Genetics
Topic: Question regarding Restriction Enzymes
Replies: 5
Views: 1578
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