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Restriction fragments

restriction fragment

(Science: molecular biology) The fragments of dNA generated by digesting DNA with a specific restriction endonuclease. Each of the fragments ends in a site recognised by that specific enzyme.


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Re: RFLPs

... left, The BB is a testcross. M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme. The bottom table shows the gel-electropohresis analyse. The table gametes ...

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by dreamtim
Wed Oct 10, 2012 8:56 pm
 
Forum: Genetics
Topic: RFLPs
Replies: 3
Views: 1411

isolation of plasmid (Midi prep)

... band. PCR verify presence of insert in vector. 2-something wrong with restriction reaction (verify if your enzymes work on different sample) 3-there ... for restriction enzyme. (unlikely but possible) 4-altered mobility of fragments due to wrong pH or fluorescent dye I dont know what can you see ...

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by triton
Mon Aug 27, 2012 4:46 pm
 
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 4445

Re:

From the gel you simply read sizes of your fragments and then check whether is it the same as expected from in silico / in papiro :) prediction of your restriction. For that you either past your sequence into some program and selects appropriate ...

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by siddharthsameer
Sun Jul 15, 2012 11:55 am
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 5891

restriction analysis

From the gel you simply read sizes of your fragments and then check whether is it the same as expected from in silico / in papiro :) prediction of your restriction. For that you either past your sequence into some program and selects appropriate ...

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by JackBean
Sun Jul 15, 2012 11:23 am
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 5891

Re: Re:

... provides only fragment size(s). Pick two DNA sequences, specify restriction sites, combine digested fragments and get a new vector <- virtual cloning. Everything done in a program(s). Main ...

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by dustman
Thu May 10, 2012 10:41 am
 
Forum: Molecular Biology
Topic: Integrated software tools
Replies: 4
Views: 1778
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