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Restriction fragments

restriction fragment

(Science: molecular biology) The fragments of dNA generated by digesting DNA with a specific restriction endonuclease. Each of the fragments ends in a site recognised by that specific enzyme.


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Souther blot and restriction mapping

Hi all, I would greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction ... gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I am struggling to find the correct ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 18

Re: RFLPs

... left, The BB is a testcross. M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme. The bottom table shows the gel-electropohresis analyse. The table gametes ...

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by dreamtim
Wed Oct 10, 2012 8:56 pm
 
Forum: Genetics
Topic: RFLPs
Replies: 3
Views: 1783

isolation of plasmid (Midi prep)

... band. PCR verify presence of insert in vector. 2-something wrong with restriction reaction (verify if your enzymes work on different sample) 3-there ... for restriction enzyme. (unlikely but possible) 4-altered mobility of fragments due to wrong pH or fluorescent dye I dont know what can you see ...

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by triton
Mon Aug 27, 2012 4:46 pm
 
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 5323

Re:

From the gel you simply read sizes of your fragments and then check whether is it the same as expected from in silico / in papiro :) prediction of your restriction. For that you either past your sequence into some program and selects appropriate ...

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by siddharthsameer
Sun Jul 15, 2012 11:55 am
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 7564

restriction analysis

From the gel you simply read sizes of your fragments and then check whether is it the same as expected from in silico / in papiro :) prediction of your restriction. For that you either past your sequence into some program and selects appropriate ...

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by JackBean
Sun Jul 15, 2012 11:23 am
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 7564
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