Restriction fragments

... constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen kit. Using the same enzymes (Kpn1 and XbaI) restriction digested the ...

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... constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen kit. Using the same enzymes (Kpn1 and XbaI) restriction digested the ...

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... lambda DNA (48.5kb) at a concentration of 55ug ml^-1 was cut with the restriction enzyme Alu I. (Lambda DNA is cut 143 times with Alu I). The following ... only wanted the addition of 20 bases of dCTP to each 3’ end of the DNA fragments. 5x tail buffer : 500mM potassium cacodylate pH 7.2, 10mM ...

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After you "cut" DNA fragments using restriction enzymes, and then are "pasting" them together using DNA ligase... do you need to ...

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If your fragments are not separated, I can't do much. But you should measure to the front of the ... you would not expect the different fragments to be the same size. It is the same with restriction sites.

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