Restriction fragments

... left, The BB is a testcross. M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme. The bottom table shows the gel-electropohresis analyse. The table gametes ...

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... band. PCR verify presence of insert in vector. 2-something wrong with restriction reaction (verify if your enzymes work on different sample) 3-there ... for restriction enzyme. (unlikely but possible) 4-altered mobility of fragments due to wrong pH or fluorescent dye I dont know what can you see ...

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From the gel you simply read sizes of your fragments and then check whether is it the same as expected from in silico / in papiro :) prediction of your restriction. For that you either past your sequence into some program and selects appropriate ...

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From the gel you simply read sizes of your fragments and then check whether is it the same as expected from in silico / in papiro :) prediction of your restriction. For that you either past your sequence into some program and selects appropriate ...

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... provides only fragment size(s). Pick two DNA sequences, specify restriction sites, combine digested fragments and get a new vector <- virtual cloning. Everything done in a program(s). Main ...

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