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Restriction fragment

restriction fragment

(Science: molecular biology) The fragments of dNA generated by digesting DNA with a specific restriction endonuclease. Each of the fragments ends in a site recognised by that specific enzyme.


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Restriction enzyme - I think my teacher made a mistake.

... a circular molecule containing a total of 5730 bp. It has sites for the restriction enzymes SstI, EcoRI and HindIII located at the following positions: ... 42 SstI position 218 HindIII positions 328 and 5518 An EcoRI/SstI fragment (1350 bp long), containing the chicken virus X ns5 gene, was cloned ...

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by TToe
Sat Nov 24, 2012 12:36 am
 
Forum: Genetics
Topic: Restriction enzyme - I think my teacher made a mistake.
Replies: 1
Views: 1193

Find number of restriction fragment size in chromosome

Hi all, I am prepare in entrance exam. I need help. A restriction endonuclease has the recognition sequence G/AATTC, where "/" indicate the cut side. This sequence is found, on average, once every 'X' residues in a chromosome. 'X' = Options ...

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by kanagasundar
Wed Nov 21, 2012 1:38 am
 
Forum: Molecular Biology
Topic: Find number of restriction fragment size in chromosome
Replies: 1
Views: 823

Restrction digest on a plasmid--how many fragments?

You are right. In a multiple cloning site, the restriction site are usually within less than20 to 30 bp of one another. Look at ... so for n plasmid you have at best 2n molecule of EtBr linked to your short fragment and someting like 500n or 1000n in the plasmid. When you adjust the sensitivity ...

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by canalon
Sat Jun 16, 2012 12:57 am
 
Forum: General Discussion
Topic: Restrction digest on a plasmid--how many fragments?
Replies: 1
Views: 1522

Re: Re:

... You pick template file, primer(s), specify conditions, run and get a fragment of certain length with expected nucleotide sequence, so you don't ... provides only fragment size(s). Pick two DNA sequences, specify restriction sites, combine digested fragments and get a new vector <- ...

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by dustman
Thu May 10, 2012 10:41 am
 
Forum: Molecular Biology
Topic: Integrated software tools
Replies: 4
Views: 1778

Integrated software tools

... things in transparent or semi-transparent way: Multiple alignment Restriction analysis Sequencing view Primer design in silico PCR Mutagenesis ... is to pick a gene, already pre-cloned in some sort of plasmid, copy a fragment with PCR, where primers have restriction sites of interest, digest/insert ...

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by dustman
Tue May 08, 2012 12:17 pm
 
Forum: Molecular Biology
Topic: Integrated software tools
Replies: 4
Views: 1778
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