Restriction fragment

RFLPs are no regions. It stands for Restriction Fragment Length Polymorphism. In short you PCR amplify a region wher you suspect there is variabilty, ...

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... variable regions in the DNA molecule, but i dont understand really what they are or how they can be useful in genotyping disease with the use of restriction enzymes or how genotyping them would work. also why can you get a blot on gel after digestion with an enzyme like EcoR1 that shows that ...

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If your fragments are not separated, I can't do much. But you should measure to the front of the ... you would not expect the different fragments to be the same size. It is the same with restriction sites.

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I have been asked to draw a restriction map that accounts for the follwing info:- the LINEAR dna is 1000bp it is cut by 4 restriction enzymes E, B, P and S the fragment sizes that are produced when these enzymes are used singly or in combination are shown ...

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so the reason you would use two restriction enzymes instead of one..is to prevent re-ligation of the dna fragments with itself instead of inserting into the plasmid..and also becos..depending on the fragment ...

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