Restriction enzymes

... can't reuse it now, can you? In fact, that is the main paradigm with enzymes. take restriction enzymes: most of them are active at 37C, but they need to be stored at -20C, and ...

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... then how can it go bad when left at RT? 2)Since I used two different restriction enzymes producing overhangs of different bases how can the vector religate, in other words ...

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... constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen kit. Using the same enzymes (Kpn1 and XbaI) restriction digested the vector- pcDNA 3.1. Then I ligated ...

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... constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen kit. Using the same enzymes (Kpn1 and XbaI) restriction digested the vector- pcDNA 3.1. Then I ligated ...

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... (keeping them open) of tubes, bottles. So work quickly when you open enzymes, primers, etc. Don’t leave a bottle open!! 8. Minimize fast motions ... need to put down, put it lid down 12. Have your own buffer stocks for restriction enzymes, PCRs etc. Don’t use the common stock. 13. Aliquot out ...

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