Restriction enzymes

... a circular molecule containing a total of 5730 bp. It has sites for the restriction enzymes SstI, EcoRI and HindIII located at the following positions: EcoRI position 42 SstI ...

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... has confused me. I am asked whether this approach, using two different restriction enzymes, could be used to generate a complete genomic library? And if not why not. It is perhaps ...

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... band. PCR verify presence of insert in vector. 2-something wrong with restriction reaction (verify if your enzymes work on different sample) 3-there is a methylation of restriction site that blocks ...

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... 500 bp. I ligated them before and yesterday I digested with related restriction enzymes for my experiment. But interestingly I saw 3 bands. One is insert(500 bp) the other ...

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You don't say what you do after restriction. Do you remove restriction enzymes? Or simply kill restrictazes by heat-shocking? Do you treat digested vector with CIAP (phosphotase)? ...

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