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Restriction enzymes

(noun) Plural form of restriction enzyme.

See restriction enzyme for definition and additional information.


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Please guide me about these restriction enzymes

For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI? please compare and describe them

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by 2angel
Tue Mar 11, 2014 7:24 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 173

Gel Electrophoresis Help!

... gel is prestained with ethidium bromide or not. what are the restriction enzymes you are using? did you check if your vector contains multiple sites of the enzymes ...

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by bravebeaker
Thu Aug 29, 2013 7:14 am
 
Forum: Molecular Biology
Topic: Gel Electrophoresis Help!
Replies: 3
Views: 901

Restriction enzyme - I think my teacher made a mistake.

... a circular molecule containing a total of 5730 bp. It has sites for the restriction enzymes SstI, EcoRI and HindIII located at the following positions: EcoRI position 42 SstI ...

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by TToe
Sat Nov 24, 2012 12:36 am
 
Forum: Genetics
Topic: Restriction enzyme - I think my teacher made a mistake.
Replies: 1
Views: 1192

First time making a genomic library

... has confused me. I am asked whether this approach, using two different restriction enzymes, could be used to generate a complete genomic library? And if not why not. It is perhaps ...

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by Awol
Mon Sep 17, 2012 10:41 am
 
Forum: Molecular Biology
Topic: First time making a genomic library
Replies: 2
Views: 1369

isolation of plasmid (Midi prep)

... band. PCR verify presence of insert in vector. 2-something wrong with restriction reaction (verify if your enzymes work on different sample) 3-there is a methylation of restriction site that blocks ...

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by triton
Mon Aug 27, 2012 4:46 pm
 
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 4445
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