Restriction enzyme

Definition

noun, plural: restriction enzymes

An enzyme that catalyzes the cleavage of DNA at restriction sites, producing small fragments used for gene splicing in recombinant DNA technology and for chromosome mapping.


Supplement

These enzymes are normally produced in bacteria and archaea as a defense mechanism against foreign DNA, especially of bacteriophages. The enzymes can be classified into Types I, II and III according to their mechanism of action.

The enzymes are named after the bacteria from where they are derived, i.e. the bacterial genus, species, strain and the order of identification. For instance, Eco RI is a restriction enzyme isolated from the bacteria: Escherichia (genus) coli (species) R (RY13 strain) I (first identified).


Also called: restriction endonuclease

Related term:



User Contributions / Comments:

Retrieved from "http://www.biology-online.org/bodict/index.php?title=Restriction_enzyme&oldid=95580"
First | Previous (Restriction endonuclease) | Next (Restriction enzymes) | Last
Please contribute to this project, if you have more information about this term feel free to edit this page.