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Restriction enzyme

Definition

noun, plural: restriction enzymes

An enzyme that catalyzes the cleavage of DNA at restriction sites, producing small fragments used for gene splicing in recombinant DNA technology and for chromosome mapping.


Supplement

These enzymes are normally produced in bacteria and archaea as a defense mechanism against foreign DNA, especially of bacteriophages. The enzymes can be classified into Types I, II and III according to their mechanism of action.

The enzymes are named after the bacteria from where they are derived, i.e. the bacterial genus, species, strain and the order of identification. For instance, Eco RI is a restriction enzyme isolated from the bacteria: Escherichia (genus) coli (species) R (RY13 strain) I (first identified).


Also called: restriction endonuclease

Related term:



User Contributions / Comments:

  • Restriction enzyme (ri STRIK shuhn EN ziem) - an enzyme that cuts double-stranded DNA into fragments by recognizing specific nucleotide sequences and cutting the DNA at those sequences.

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Results from our forum


MOLECULAR CLONING

... of the oligo will form covalent bond with the 5'-phosphate end of the restriction enzyme-digested vector end, but the 5'-OH end of the oligo will have a nick next to the 3'-OH ...

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 4104

PFGE with Haemophilus influenzae

I am doing PFGE with Haemophilus influenzae. Also i am using SmaI restriction enzyme, but i can not get result. Not as smear, actually i am counting 6 bands but as we know ...

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by SMG
Sun May 05, 2013 3:07 pm
 
Forum: Molecular Biology
Topic: PFGE with Haemophilus influenzae
Replies: 1
Views: 730

Restriction enzyme mapping

Is this correct? Second question. http://www.flickr.com/photos/85189286@N06/8220858932/in/photostream

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by TToe
Mon Nov 26, 2012 12:34 pm
 
Forum: Genetics
Topic: Restriction enzyme mapping
Replies: 1
Views: 691

Restriction enzyme - I think my teacher made a mistake.

... a circular molecule containing a total of 5730 bp. It has sites for the restriction enzymes SstI, EcoRI and HindIII located at the following positions: EcoRI position 42 SstI ...

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by TToe
Sat Nov 24, 2012 12:36 am
 
Forum: Genetics
Topic: Restriction enzyme - I think my teacher made a mistake.
Replies: 1
Views: 1192

Re: RFLPs

... M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme. The bottom table shows the gel-electropohresis analyse. The table gametes shows the ...

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by dreamtim
Wed Oct 10, 2012 8:56 pm
 
Forum: Genetics
Topic: RFLPs
Replies: 3
Views: 1411
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