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Restriction endonuclease

Definition

noun, plural: restriction enzymes

An endonuclease that cleaves DNA at specific sites, producing small fragments used for gene splicing in recombinant DNA technology and for chromosome mapping.


Supplement

These enzymes are normally produced in bacteria and archaea as a defense mechanism against foreign DNA, especially of bacteriophages. The enzymes can be classified into Types I, II and III according to their mechanism of action.

The enzymes are named after the bacteria from where they are derived, i.e. the bacterial genus, species, strain and the order of identification. For instance, Eco RI is a restriction enzyme isolated from the bacteria: Escherichia (genus) coli (species) R (RY13 strain) I (first identified).


Also called: restriction enzyme


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Souther blot and restriction mapping

Hi all, I would greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 22

Restriction Digesion

which is the best option for Restriction Endonuclease digesion ??? A) 37' C water bath B) 37' C PCR program

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by Helics
Sun Apr 06, 2014 4:07 pm
 
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 3
Views: 929

Find number of restriction fragment size in chromosome

Hi all, I am prepare in entrance exam. I need help. A restriction endonuclease has the recognition sequence G/AATTC, where "/" indicate the cut side. This ...

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by kanagasundar
Wed Nov 21, 2012 1:38 am
 
Forum: Molecular Biology
Topic: Find number of restriction fragment size in chromosome
Replies: 1
Views: 1109

Recombinant DNA not degraded inside transformed cells, why?

... DNA enters a bacterial cell, there is a race between methylases and restriction endonucleases. If the DNA is methylated as "self" before the corresponding endonuclease ...

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by jonmoulton
Thu Sep 27, 2012 4:26 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 4858

genetic engineering- not understanding some concepts

... have the same methylation sequence and cuts it via host controlled restriction?? 3. In genetic engineering, when we want to clone a gene, I ... are any good sites for solving questions about ligation, restriction endonuclease, blunt and sticky ends and pUC8 plasmids and whatnot. If you ...

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by radioinahole
Fri Oct 21, 2011 2:38 pm
 
Forum: Molecular Biology
Topic: genetic engineering- not understanding some concepts
Replies: 2
Views: 1826
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