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Restriction

restriction

1. The process with which foreign dNA that has been introduced into a prokaryotic cell becomes ineffective.

2. A limitation.


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5' LTR sequece

You may try to find comparable restriction endoneucleases for wild type 5'LTR. Hybrid 5'LTR generates different size RE digest products.

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by Helics
Fri Aug 01, 2014 6:07 pm
 
Forum: Molecular Biology
Topic: 5' LTR sequece
Replies: 1
Views: 280

Restriction Digesion

which is the best option for Restriction Endonuclease digesion ??? A) 37' C water bath B) 37' C PCR program

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by Helics
Sun Apr 06, 2014 4:07 pm
 
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 3
Views: 691

Please guide me about these restriction enzymes

For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI? please compare and describe them

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by 2angel
Tue Mar 11, 2014 7:24 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 1087

Free online software tools for molecular cloning

... where you can find a couple of browser-based tools facilitating your everyday cloning work. At this time a DNA sequence editor and a restriction analysis tool are available. I have also some other ideas that may evolve into additional tools in future. I will much appreciate any feedback ...

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by vladCH
Sat Feb 22, 2014 8:12 pm
 
Forum: Molecular Biology
Topic: Free online software tools for molecular cloning
Replies: 0
Views: 1008

Annealing Temparature

The primers obviously contain added restriction sites and the matching part is quite short. I would anneal the first cycle at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. ...

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 1368
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