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Restriction

restriction

1. The process with which foreign dNA that has been introduced into a prokaryotic cell becomes ineffective.

2. A limitation.


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Souther blot and restriction mapping

Hi all, I would greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 21

Gene Upregulation/Increased Expression in HMG-CoA Reductase

... of the enzyme, and that's where I'm getting confused. Citation: Mutungi G, Torres-gonzalez M, Mcgrane MM, Volek JS, Fernandez ML. Carbohydrate restriction and dietary cholesterol modulate the expression of HMG-CoA reductase and the LDL receptor in mononuclear cells from adult men. Lipids Health ...

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by Upperninety8
Tue Sep 30, 2014 3:21 am
 
Forum: Genetics
Topic: Gene Upregulation/Increased Expression in HMG-CoA Reductase
Replies: 0
Views: 47

5' LTR sequece

You may try to find comparable restriction endoneucleases for wild type 5'LTR. Hybrid 5'LTR generates different size RE digest products.

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by Helics
Fri Aug 01, 2014 6:07 pm
 
Forum: Molecular Biology
Topic: 5' LTR sequece
Replies: 1
Views: 486

Restriction Digesion

which is the best option for Restriction Endonuclease digesion ??? A) 37' C water bath B) 37' C PCR program

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by Helics
Sun Apr 06, 2014 4:07 pm
 
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 3
Views: 929

Please guide me about these restriction enzymes

For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI? please compare and describe them

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by 2angel
Tue Mar 11, 2014 7:24 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 1337
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