Dictionary » R » Restriction

Restriction

restriction

1. The process with which foreign dNA that has been introduced into a prokaryotic cell becomes ineffective.

2. A limitation.


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Restriction enzyme mapping

Is this correct? Second question. http://www.flickr.com/photos/85189286@N06/8220858932/in/photostream

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by TToe
Mon Nov 26, 2012 12:34 pm
 
Forum: Genetics
Topic: Restriction enzyme mapping
Replies: 1
Views: 332

Restriction enzyme - I think my teacher made a mistake.

The vector is a circular molecule containing a total of 5730 bp. It has sites for the restriction enzymes SstI, EcoRI and HindIII located at the following positions: EcoRI position 42 SstI position 218 HindIII positions 328 and 5518 An EcoRI/SstI fragment (1350 bp ...

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by TToe
Sat Nov 24, 2012 12:36 am
 
Forum: Genetics
Topic: Restriction enzyme - I think my teacher made a mistake.
Replies: 1
Views: 451

Find number of restriction fragment size in chromosome

Hi all, I am prepare in entrance exam. I need help. A restriction endonuclease has the recognition sequence G/AATTC, where "/" indicate the cut side. This sequence is found, on average, once every 'X' residues in a chromosome. 'X' = Options ...

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by kanagasundar
Wed Nov 21, 2012 1:38 am
 
Forum: Molecular Biology
Topic: Find number of restriction fragment size in chromosome
Replies: 1
Views: 259

Re: separation problem with GelRed

Sadly I did not help us out. In our lab often we want to check early if we have good product/restriction pattern. And with GelRed gels have to run longer to get nice bands. But it helps indeed if you run longer. Then result can be nice.

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by citroenboom
Wed Nov 14, 2012 1:41 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 11
Views: 12170

rflp pcr

WELL IT VERY GOOD QUESTION. RESTRICTION FREGMENT LENGTH POLYMORPHISM HAS AN ENOROMOUS ROLL ON MOLECULAR BIOLOGY. IT IS USED TO STUDY THE POLYMORPIC NATURE OF ANY DNA SEQUENCE. BESIDES THIS IT IS ALSO USED IN FORENSIC SCIENCE FOR DNA TYPING.

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by Helics
Wed Oct 17, 2012 3:34 pm
 
Forum: Molecular Biology
Topic: rflp pcr
Replies: 1
Views: 439
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