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Dictionary » R » Restriction

Restriction

restriction

1. The process with which foreign dNA that has been introduced into a prokaryotic cell becomes ineffective.

2. A limitation.


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Please guide me about these restriction enzymes

For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI? please compare and describe them

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by 2angel
Tue Mar 11, 2014 7:24 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 174

Free online software tools for molecular cloning

... where you can find a couple of browser-based tools facilitating your everyday cloning work. At this time a DNA sequence editor and a restriction analysis tool are available. I have also some other ideas that may evolve into additional tools in future. I will much appreciate any feedback ...

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by vladCH
Sat Feb 22, 2014 8:12 pm
 
Forum: Molecular Biology
Topic: Free online software tools for molecular cloning
Replies: 0
Views: 139

Annealing Temparature

The primers obviously contain added restriction sites and the matching part is quite short. I would anneal the first cycle at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. ...

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 350

Why must we avert the genome of Eden?

... and integrity (for the sake of the analogy put aside creativity and intelligence during the copying process). But let's say we introduce a basic restriction: in order to make a copy one must find two independent but compatible sources. Now even after 10000 copies the final copy will more likely ...

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by Hunor
Mon Sep 23, 2013 12:08 am
 
Forum: Evolution
Topic: Why must we avert the genome of Eden?
Replies: 6
Views: 3028

Gel Electrophoresis Help!

size of your vector/insert. gel is prestained with ethidium bromide or not. what are the restriction enzymes you are using? did you check if your vector contains multiple sites of the enzymes you are using? The bands are way up, higher than 10k bp..

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by bravebeaker
Thu Aug 29, 2013 7:14 am
 
Forum: Molecular Biology
Topic: Gel Electrophoresis Help!
Replies: 3
Views: 901
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