Rescue work

It looks like the problem is with figuring out what is the use of your work. DNA sequencing of a species can yield quite a bit of new knowledge. ... Does RNAi work? Can you test if gene homologs in this organism can rescue a mutant phenotype in another (well-known) species? How about a smaller ...

See entire post

... and would like to express them in yeast to see if they can partially rescue an arsenic-sensitive yeast strain. I am wondering how I choose an ... it might help to talk to a person :) If it helps the yeast strains I am working with are the following: W303-1A MATaura3-1 leu2-3/112 trp1-1 his3-11/15 ...

See entire post

... Morpholino experiment. These are the five mispair experiment, the mRNA rescue experiment and the two-nonoverlapping-oligo experiment. The five mispair ... wild-type condition. The mRNA rescue experiment is beautiful when it works and, when it works, is an excellent proof of specificity. Unfortunately, ...

See entire post

... co-injected ATG-MO 1.5ng together with p53MO 5ng. Necrosis in brain is rescued by coinjection with p53, however CE phenotype can not be rescue. ... off-target interaction. The other possibility is my splicing MO did not work at all.( I injected with 10ng, still I could not see any phenotype.) ...

See entire post

Snowcapk, As far as I am concerned the gold standard is rescue. One way is to make mRNA using the Mmassage Mmachine kit and inject with ... effect on the target gene. However, more often than not this will not work, there are various reasons for this including that you are injecting a large ...

See entire post