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Rescue

rescue

1. The act of rescuing; deliverance from restraint, violence, or danger; liberation. Spur to the rescue of the noble talbot. (Shak)

2. The forcible retaking, or taking away, against law, of things lawfully distrained. The forcible liberation of a person from an arrest or imprisonment.

The retaking by a party captured of a prize made by the enemy. The rescue of a prisoner from the court is punished with perpetual imprisonment and forfeiture of goods. (Blackstone) Rescue grass. [Etymol. Uncertain.

(Science: botany) A tall grass (Ceratochloa unioloides) somewhat resembling chess, cultivated for hay and forage in the southern states.

Origin: From Rescue,; cf. Rescous.


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I have a problem seeing the bigger picture of research

... any form of genetics to create mutants to study individual gene function? Does RNAi work? Can you test if gene homologs in this organism can rescue a mutant phenotype in another (well-known) species? How about a smaller piece of the gene, eg. a specific protein domain, whether it rescues ...

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by FlyMusings
Sat Sep 29, 2012 5:14 pm
 
Forum: General Discussion
Topic: I have a problem seeing the bigger picture of research
Replies: 2
Views: 1319

Choosing a yeast vector

Hello, I have cloned several plant arsenic transporters and would like to express them in yeast to see if they can partially rescue an arsenic-sensitive yeast strain. I am wondering how I choose an expression vector that is compatible with my yeast strains. Have done a lot of internet ...

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by alyson2324
Thu Aug 02, 2012 4:54 pm
 
Forum: Molecular Biology
Topic: Choosing a yeast vector
Replies: 0
Views: 1217

Re: genomic rescue construct from BAC DNA

If I were you, I wouldn't just add more DNA to the reaction. I've come to believe that if you can see it, you can clone it. Are you using the right buffer for your restriction enzymes? For Fermentas enzymes, they suggest 2x tango but the BamHI is reduced to 50-100% activity. What company are the enz...

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by antonyviridae
Wed Sep 07, 2011 8:33 pm
 
Forum: Molecular Biology
Topic: genomic rescue construct from BAC DNA
Replies: 2
Views: 3517

genomic rescue construct from BAC DNA

Hi, I am currently generating a genomic rescue construct, and I need some help because it is not working. No-one in my lab has done this before and I don't want to waste all my time re-inventing the wheel. If you have done this before, please ...

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by GAV65
Wed Aug 24, 2011 5:08 am
 
Forum: Molecular Biology
Topic: genomic rescue construct from BAC DNA
Replies: 2
Views: 3517

genomic rescue construct to use in drosophila

I am really confused as to how do you go about making a genomic rescue construct and how you make the transgenic fly. I am interested in making a rescue construct for a lethal gene that is really huge.

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by g8824u
Wed Jul 13, 2011 8:44 pm
 
Forum: Genetics
Topic: genomic rescue construct to use in drosophila
Replies: 0
Views: 1206
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