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Reproducibility

reproducibility

1. Ability to cause to exist again or to present again.

2. The ability to duplicate measurements over long periods of time by different laboratories.


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Re: ChIP-Seq for small number of cells

... However, I can get only a limited number of cells per mouse (~3000/mouse). So I'd end up with 20,000 cells, is that enough for ChIP-seq? How about reproducibility? I read the paper from the Myers' lab, they used 50ng DNA and amplified it. 20,000 cells should give me ~200ng DNA, that might work. ...

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by fizza9
Thu Aug 18, 2011 11:54 am
 
Forum: Genetics
Topic: ChIP-Seq for small number of cells
Replies: 4
Views: 3869

Problems with Evolution !

... are several animal models, but no single model is completely satisfactory. One model is the LP-BM5 mouse. Primary advantages of this model are its reproducibility in the laboratory, its cost-effectiveness, and the similarity of many of the observed defects to human AIDS. The major disadvantage ...

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by JorgeLobo
Tue Mar 03, 2009 11:16 am
 
Forum: Evolution
Topic: Problems with Evolution !
Replies: 33
Views: 21788

ELISA question

I'm doing MIP-2 ELISA for a cell line using a kit from Biosource (now part of Invitrogen). Reproducibility is bad in terms of the equations obtained from the standard curves and the values obtained between different experiments. The big intercepts (big blank readings) ...

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by hanhan2008
Fri Apr 25, 2008 5:02 pm
 
Forum: Molecular Biology
Topic: ELISA question
Replies: 1
Views: 1078

accuracy and reproducibilty

The one with low SD would give you internal consistency, meaning that if it works for you once, it'll probably work again. However if it has low reproducibility, then it's probably off, meaning if you measure out 200uL, you might consistently get only 180uL on average. Therefore the values you ...

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by mith
Fri Mar 07, 2008 1:21 pm
 
Forum: General Discussion
Topic: accuracy and reproducibilty
Replies: 3
Views: 1758

accuracy and reproducibilty

... is reflected by scale measurements, that is your mean mass does not change each time--the scale isn't just reading off random numbers. This is reproducibility.

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by mith
Fri Mar 07, 2008 12:09 pm
 
Forum: General Discussion
Topic: accuracy and reproducibilty
Replies: 3
Views: 1758
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