Repressor gene

cis and trans are two terms generally used in genetics. Cis means that it must be on the same strand in order to ... the same DNA strand(of course it can still be, it just isn't a requirement). The repressor gene acts in trans. It doesn't really matter where it is located, as long ...

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cis and trans elements are mostly used in regard with repressor gene cis if the repressor gene is on the same dna strand trans if it is on another one

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... start site. A cis-acting element must act on the same segment of "gene" (it usually is DNA, but it can be RNA) to which it is itself a ... If the "binding site" is something other than a promoter or repressor binding site, then whatever process this is, must have some sense ...

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Sorry, the other half of the question: You don't need to amplify the gene sequence after you've cloned it in order to express it. You clone the ... of the sequence. Induction is turning on gene expression by removing a repressor most typically, though there are probably other ways to induce.

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There are repressible and inducible systems. In an inducible system, repressor is normally bound to the gene or operon (=> no expression) until effector molecule binds it causing the release from ...

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