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Reporter gene

reporter gene

(Science: molecular biology) A gene that encodes an easily assayed product (e.g. CAT) that is coupled to the upstream sequence of another gene and transfected into cells. The reporter gene can then be used to see which factors activate response elements in the upstream region of the gene of interest.


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I have got some BIG issues! forgive the long post

... further ado: Here they are: Indirect DNA testing : I know yo target the genes that are in severe proximity to our targeted gene and that you make use a fluorescent nucleotide or a tagged reporter molecule that will attach themselves to those genes and fluorescent in order ...

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by saddy51
Sun Jan 04, 2015 10:39 am
 
Forum: Genetics
Topic: I have got some BIG issues! forgive the long post
Replies: 1
Views: 1918

Re: Any ideas for vaccine development or anti-viral drugs???

... DK, Pratt WD, Blouch R, Stein DA, Aman MJ, Iversen PL, Bavari S. Gene-Specific Countermeasures against Ebola Virus Based on Antisense Phosphorodiamidate ... additional VP24-specific PMOs to reduce cell-free translation of a VP24 reporter, to inhibit in vitro replication of Ebolavirus, and to protect ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12742

Transcrption factor-promoter (cis-element) interaction

... with plant cells and the most common and easy way I guess is the reporter gene assay. the promoter in question is designed in a vector carrying the GUS reporter gene ...

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by bravebeaker
Thu Aug 22, 2013 3:31 am
 
Forum: Molecular Biology
Topic: Transcrption factor-promoter (cis-element) interaction
Replies: 3
Views: 1952

Orientation of genes

"to be cloned" part is not needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen ...

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by Sue4
Mon Jan 28, 2013 9:03 pm
 
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 1484

Orientation of genes

When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired ...

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by Sue4
Mon Jan 28, 2013 8:23 pm
 
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 1484
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