Reporter gene

... and start codon in between the two coding sequences just incase the genes should spit, which there is a chance of if you're trying to splice ... larger sequence or if it is just a plasmid. because then you might have reporters getting made without the protein of interest (false reports) or ...

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Let me continue with molecular cloning. When you generate N-fusion proteins (our "gene" of interest is fused to the N-term to the reporter gene), does it matter if you leave the Kozak sequence and a start codon in between the two ...

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I'm trying to create a pspA reporter plasmid by linking the psp promoter using the thi- genotype of E.coli MC4100 as an auxotrophic marker, however I'm having 'a little' trouble finding out which gene has actually been deleted or mutated so that I can incorporate it into my plasmid. ...

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In general you should include some sort of reporter gene on your construct, and then see the outcome when you cultivate your microbe - e.g. the colour of your ...

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... mentioned (rescue through coinjection with a construct containing the gene's coding region in its native regulatory context, e.g. a BAC) and it ... the construct is a BAC (requiring homologous recombination to insert a reporter) or even just a small fragment, because there must then be additional ...

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