Reporter gene

"to be cloned" part is not needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen ...

See entire post

When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired ...

See entire post

I know that a reporter gene can be used to detect the foreign gene expression if it is fused with the foreign gene. But can we monitor ...

See entire post

... DNA binding transcription factor has a direct interaction with a gene of interest ( we know that the TF has impact on expression because a ... this, but I can't come up with anything useful. Perhaps some kind of reporter gene assay might work? But reporter genes can't tell me what (if ...

See entire post

Hi all, I am doing some mutation analysis. I have generated 4 mutants and a wild type and have been trying to transfect my cells ... we are getting constitutive silencing, this even happens when using a GFP reporter, where there is no phenotype. We can maintain expression (as detected ...

See entire post