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Reporter gene

reporter gene

(Science: molecular biology) A gene that encodes an easily assayed product (e.g. CAT) that is coupled to the upstream sequence of another gene and transfected into cells. The reporter gene can then be used to see which factors activate response elements in the upstream region of the gene of interest.


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Transcrption factor-promoter (cis-element) interaction

... with plant cells and the most common and easy way I guess is the reporter gene assay. the promoter in question is designed in a vector carrying the GUS reporter gene ...

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by bravebeaker
Thu Aug 22, 2013 3:31 am
 
Forum: Molecular Biology
Topic: Transcrption factor-promoter (cis-element) interaction
Replies: 3
Views: 1199

Orientation of genes

"to be cloned" part is not needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen ...

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by Sue4
Mon Jan 28, 2013 9:03 pm
 
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 1139

Orientation of genes

When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired ...

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by Sue4
Mon Jan 28, 2013 8:23 pm
 
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 1139

Reporter gene without fusion

I know that a reporter gene can be used to detect the foreign gene expression if it is fused with the foreign gene. But can we monitor ...

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by Sue4
Fri Jan 25, 2013 11:43 am
 
Forum: Molecular Biology
Topic: Reporter gene without fusion
Replies: 1
Views: 1101

Direct or indirect protein-DNA interaction

... DNA binding transcription factor has a direct interaction with a gene of interest ( we know that the TF has impact on expression because a ... this, but I can't come up with anything useful. Perhaps some kind of reporter gene assay might work? But reporter genes can't tell me what (if ...

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by nukeman
Wed Oct 31, 2012 5:21 pm
 
Forum: Molecular Biology
Topic: Direct or indirect protein-DNA interaction
Replies: 1
Views: 1016
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