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Replication fork

Replication fork

A Y-shaped region in a chromosome that serves as the growing site for dna replication.

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Scientific paper on DNA replication & gel electrophoresis

... -They inserted two terB sites to get a one unidirectional replication system -Only the minimal protein required to sustain oriC and ... that migrated to the top of the gel indicate a stalled replication fork. This was the bulk reaction. -Then used gel-filtration chromatography ...

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by dekk1525
Sat Sep 08, 2012 11:17 pm
Forum: Molecular Biology
Topic: Scientific paper on DNA replication & gel electrophoresis
Replies: 0
Views: 3960

Re: Generation of DNA nucleotides

... ignores them altogether. http://www.hhmi.org/biointeractive/media/DNAi_replication_vo2-lg.mov >Talking of transcription (similar process) this ... multiple processing. Most web sites show only a single replication fork. One claims there are hundreds of concurrent DNA replication activities ...

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by gs99
Sun Jan 10, 2010 2:19 am
Forum: Molecular Biology
Topic: Generation of DNA nucleotides
Replies: 65
Views: 51492

How do they do it?

Briefly, what comes to DNA and replication ( E. coli as an example model): there are several enzymes involved in the ... on the strand. Next, other enzyme components form the replication fork. Several helicases and topoisomerases are involved here. Only after this DNA polymerase ...

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by biohazard
Fri Oct 31, 2008 9:16 pm
Forum: Molecular Biology
Topic: How do they do it?
Replies: 11
Views: 10025

3 stages of DNA replication

Initiation (starting point of replication) --> Elongation (of new DNA at replication fork) --> Termination

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by honee_v
Mon Jan 29, 2007 1:02 am
Forum: Cell Biology
Topic: 3 stages of DNA replication
Replies: 3
Views: 14542

bidimensional electrophoresis

... had to do with electrophoresis of Replicating DNA (at an origin of replication/replication fork). During the first electrophoresis, the DNA fragments (Forks) were run through a gel that ...

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by LilKim
Tue Jun 20, 2006 2:32 am
Forum: Molecular Biology
Topic: bidimensional electrophoresis
Replies: 7
Views: 2385
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