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Replica plating

replica plating

(Science: technique) technique for testing the genetic characteristics of bacterial colonies. A dilute suspension of bacteria is first spread, in a petri dish, on agar containing a medium expected to support the growth of all bacteria, the master plate. Each bacterial cell in the suspension is expected to give rise to a colony. A sterile velvet pad, the same size as the petri dish, is then pressed onto it, picking up a sample of each colony. The bacteria can then be stamped onto new sterile petri dishes, plates, in the identical arrangement. The media in the new plates can be made up to lack specific nutritional requirements or to contain antibiotics. Thus colonies can be identified that cannot grow without specific nutrients or that are antibiotic resistant and cells with mutations in particular genes can be isolated.


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Screening of a mutation?

... become unable to synthesize thiamine from scratch, you will have to do replica plating on MM and MM+Thiamine. If a clone grow only on the supplemented plate, you know that ...

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by canalon
Tue Feb 01, 2011 10:25 pm
 
Forum: Genetics
Topic: Screening of a mutation?
Replies: 2
Views: 1516

mutation

If you are looking for auxotrophs, replica plating is the best. You can inoculate a lot of isolates in several diff media (like minimal medium, etc) ...

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by mkwaje
Sun Jan 31, 2010 3:41 pm
 
Forum: Molecular Biology
Topic: mutation
Replies: 3
Views: 1569

mutation

Does replica plating method help to identify that mutation has occured?

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by ishmit
Mon Oct 12, 2009 5:12 pm
 
Forum: Molecular Biology
Topic: mutation
Replies: 3
Views: 1569

Don't understand replica plating...

... were grown in a medium containing just glucose. Then, some of the cells from each colony were transferred to medium with just lactose, i.e., the replica plate. Subsequently, the cells that differed from the replica plate that were on the master (original) plate were unable to metabolize lactose. ...

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by WiseIdiot
Sat Nov 08, 2008 8:06 pm
 
Forum: Cell Biology
Topic: Don't understand replica plating...
Replies: 1
Views: 1514


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