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Replica plate

replica plate

A technique in which the pattern of bacterial colonies on a culture plate is copied using sterile filter paper, and then the paper is pressed against a second sterile plate. The new plate is infected with cells in the same relative positions as the colonies in the original plate. Usually the new plate is tested quite destructively for some property. The ones with the best result are identified, and the corresponding group of organisms on the original plate can be identified because they are in the equivalent place.


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Screening of a mutation?

The plates should be a defined Minimal medium (MM) of course (like M9 glucose) But I am slightly ... that suddenly become unable to synthesize thiamine from scratch, you will have to do replica plating on MM and MM+Thiamine. If a clone grow only on the supplemented plate, you ...

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by canalon
Tue Feb 01, 2011 10:25 pm
 
Forum: Genetics
Topic: Screening of a mutation?
Replies: 2
Views: 1469

Don't understand replica plating...

... each colony were transferred to medium with just lactose, i.e., the replica plate. Subsequently, the cells that differed from the replica plate that were on the master ...

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by WiseIdiot
Sat Nov 08, 2008 8:06 pm
 
Forum: Cell Biology
Topic: Don't understand replica plating...
Replies: 1
Views: 1486

Help

... SS Gg d) Ss GG e) SS GG I DONT KNOW WAT THE SYMBOLS MEAN? 7) A single plate of E. coli was replicated onto several plates which were later treated with T1 phage. These plates grew phage ...

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by chousta
Sat Aug 23, 2008 7:14 am
 
Forum: Genetics
Topic: Help
Replies: 1
Views: 3194


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