Dictionary » R » Renaturation



noun, plural: renaturations

(molecular biology) The conversion of denatured protein or nucleic acid to its native configuration


Renaturation in molecular biology refers to the reconstruction of a protein or nucleic acid (such as DNA) to their original form especially after denaturation. This process is therefore the inverse of denaturation. In denaturation, the proteins or nucleic acids lose their native biomolecular structure. For instance, a DNA molecule is denatured through heating. The heat-denatured DNA separates into two strands. As for proteins, denaturation is carried out by the application of an external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent, radiation, or heat.1. Reconstructing the denatured nucleic acid or protein into its original form is done by the process of renaturation. For instance, a heat-denatured DNA can revert to its original form by cooling slowly the two strands and then reform into its original double-stranded helix. A denatured protein may be restored following denaturation although it is not as common as it can be done on denatured nucleic acids. One way through which a denatured protein is restored to its original form is by removing the SDS and denaturing agents following denaturation during PAGE or IEF protein identification.2

See also:

1 Denaturation (biochemistry). (n.d.) Mosby's Medical Dictionary, 8th edition. (2009). Retrieved March 23 2015 from http://medical-dictionary.thefreedictionary.com/Denaturation+(biochemistry).
2 Rosenberg, I. (2005). Protein analysis and purification benchtop techniques. Boston: Birkhäuser.

Please contribute to this project, if you have more information about this term feel free to edit this page

Results from our forum

Denaturation and refolding of an enzyme

... but dont get the question and not sure about the answer too, if i am exactly answering the question? Q2.What is the function of cysteine in the renaturation reaction? I think the answer : The side chain on cysteine is thiol participates in enzymatic reactions and act as nucleophile. Cysteine ...

See entire post
by enzsel
Thu Dec 02, 2010 1:54 pm
Forum: Cell Biology
Topic: Denaturation and refolding of an enzyme
Replies: 3
Views: 4123


... is heated to 100degrees to separate the strands and then allowed to cool slowly the two strands can find each other and reform the double helix (renaturation.) Consider the following DNA molecule: 5' GCGCGCGCGCGCGC 3' 3' CGCGCGCGCGCGCG 5' If this DNA is heated to 100degrees what would the structure ...

See entire post
by EdZehtab
Thu Nov 04, 2010 4:05 pm
Forum: Genetics
Topic: DNA Questions *PLEASE HELP*
Replies: 5
Views: 6739

help !about DNA renaturation and repetitive sequence

why does the speed of DNA renaturation accelerate as the mount of repetitive sequence ? i want know the mechanism of this phenomenon. help

See entire post
by yissa
Fri Mar 26, 2010 4:06 pm
Forum: General Discussion
Topic: help !about DNA renaturation and repetitive sequence
Replies: 2
Views: 1323

renaturation kinetics

What are the classes of DNA sequences in genomic DNA (based on renaturation kinetics)?

See entire post
by HelloKitty
Tue Sep 22, 2009 2:53 am
Forum: Molecular Biology
Topic: renaturation kinetics
Replies: 0
Views: 1219

Re: Denaturation of enzymes

So in response to what poison wrote, I need a little more elaboration upon the denaturation and renaturation. You said that most proteins don't reform their natural shape. Could this be due to the fact that you need chaperones and other such helpers to achieve a proteins ...

See entire post
by carmenh
Fri Feb 27, 2009 10:21 pm
Forum: General Discussion
Topic: Denaturation of enzymes
Replies: 3
Views: 103791
View all matching forum results

This page was last modified 23:23, 31 March 2015. This page has been accessed 22,786 times. 
What links here | Related changes | Permanent link