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Renaturation

renaturation

(Science: molecular biology) The conversion of denatured protein or dNA to its native configuration.

This is rare for proteins. However, if dNA is denatured by heating the two strands separate and if the heat denatured DNA is then cooled slowly the double stranded helix reforms.


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Denaturation and refolding of an enzyme

... but dont get the question and not sure about the answer too, if i am exactly answering the question? Q2.What is the function of cysteine in the renaturation reaction? I think the answer : The side chain on cysteine is thiol participates in enzymatic reactions and act as nucleophile. Cysteine ...

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by enzsel
Thu Dec 02, 2010 1:54 pm
 
Forum: Cell Biology
Topic: Denaturation and refolding of an enzyme
Replies: 3
Views: 3517

DNA Questions *PLEASE HELP*

... is heated to 100degrees to separate the strands and then allowed to cool slowly the two strands can find each other and reform the double helix (renaturation.) Consider the following DNA molecule: 5' GCGCGCGCGCGCGC 3' 3' CGCGCGCGCGCGCG 5' If this DNA is heated to 100degrees what would the structure ...

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by EdZehtab
Thu Nov 04, 2010 4:05 pm
 
Forum: Genetics
Topic: DNA Questions *PLEASE HELP*
Replies: 5
Views: 6150

help !about DNA renaturation and repetitive sequence

why does the speed of DNA renaturation accelerate as the mount of repetitive sequence ? i want know the mechanism of this phenomenon. help

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by yissa
Fri Mar 26, 2010 4:06 pm
 
Forum: General Discussion
Topic: help !about DNA renaturation and repetitive sequence
Replies: 2
Views: 1185

renaturation kinetics

What are the classes of DNA sequences in genomic DNA (based on renaturation kinetics)?

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by HelloKitty
Tue Sep 22, 2009 2:53 am
 
Forum: Molecular Biology
Topic: renaturation kinetics
Replies: 0
Views: 1155

Re: Denaturation of enzymes

So in response to what poison wrote, I need a little more elaboration upon the denaturation and renaturation. You said that most proteins don't reform their natural shape. Could this be due to the fact that you need chaperones and other such helpers to achieve a proteins ...

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by carmenh
Fri Feb 27, 2009 10:21 pm
 
Forum: General Discussion
Topic: Denaturation of enzymes
Replies: 3
Views: 97658
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