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Renaturation

Definition

noun, plural: renaturations

(molecular biology) The conversion of denatured protein or nucleic acid to its native configuration


Supplement

Renaturation in molecular biology refers to the reconstruction of a protein or nucleic acid (such as DNA) to their original form especially after denaturation. This process is therefore the inverse of denaturation. In denaturation, the proteins or nucleic acids lose their native biomolecular structure. For instance, a DNA molecule is denatured through heating. The heat-denatured DNA separates into two strands. As for proteins, denaturation is carried out by the application of an external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent, radiation, or heat.1. Reconstructing the denatured nucleic acid or protein into its original form is done by the process of renaturation. For instance, a heat-denatured DNA can revert to its original form by cooling slowly the two strands and then reform into its original double-stranded helix. A denatured protein may be restored following denaturation although it is not as common as it can be done on denatured nucleic acids. One way through which a denatured protein is restored to its original form is by removing the SDS and denaturing agents following denaturation during PAGE or IEF protein identification.2


See also:

Reference(s):
1 Denaturation (biochemistry). (n.d.) Mosby's Medical Dictionary, 8th edition. (2009). Retrieved March 23 2015 from http://medical-dictionary.thefreedictionary.com/Denaturation+(biochemistry).
2 Rosenberg, I. (2005). Protein analysis and purification benchtop techniques. Boston: Birkhäuser.


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Denaturation and refolding of an enzyme

... but dont get the question and not sure about the answer too, if i am exactly answering the question? Q2.What is the function of cysteine in the renaturation reaction? I think the answer : The side chain on cysteine is thiol participates in enzymatic reactions and act as nucleophile. Cysteine ...

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by enzsel
Thu Dec 02, 2010 1:54 pm
 
Forum: Cell Biology
Topic: Denaturation and refolding of an enzyme
Replies: 3
Views: 4123

DNA Questions *PLEASE HELP*

... is heated to 100degrees to separate the strands and then allowed to cool slowly the two strands can find each other and reform the double helix (renaturation.) Consider the following DNA molecule: 5' GCGCGCGCGCGCGC 3' 3' CGCGCGCGCGCGCG 5' If this DNA is heated to 100degrees what would the structure ...

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by EdZehtab
Thu Nov 04, 2010 4:05 pm
 
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Topic: DNA Questions *PLEASE HELP*
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help !about DNA renaturation and repetitive sequence

why does the speed of DNA renaturation accelerate as the mount of repetitive sequence ? i want know the mechanism of this phenomenon. help

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by yissa
Fri Mar 26, 2010 4:06 pm
 
Forum: General Discussion
Topic: help !about DNA renaturation and repetitive sequence
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renaturation kinetics

What are the classes of DNA sequences in genomic DNA (based on renaturation kinetics)?

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by HelloKitty
Tue Sep 22, 2009 2:53 am
 
Forum: Molecular Biology
Topic: renaturation kinetics
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Re: Denaturation of enzymes

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by carmenh
Fri Feb 27, 2009 10:21 pm
 
Forum: General Discussion
Topic: Denaturation of enzymes
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