Regions

... conserved, while some loops on surface of the protein will be divergent. Both on DNA and amino acid level. You can design primers on the divergent regions and then they will be species-specific, because they will bind to the sequence of only one species. On the other hand, you can design primers ...

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I think this is a case where winding the DNA onto histones protects it from the nuclease, so the DNA is cut only in regions between the histones. If the same length of DNA is wound around each histone and every DNA segment between histones gets cut, the fragments after inter-histone ...

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There are many theories and contributing factors, but, most importantly, when a cell divides by mitosis, regions of the chromosomes called telomeres are lost if the telomerase gene is not activated, which plugs and repairs these telomeres (which is why cells have a Hayflick ...

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... marker, we'll just have a difficult time screening for our mutant. One interesting detail: the GC content of the upstream and downstream flanking regions used to recombine with the flanking regions of our gene of interest for deletion is quite different. The upstream region has ~28% GC, while ...

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... vector (gentamicin resistance, SacB gene). My vector contains ~1.2kb of homologous sequences that flank both sides of my gene (~2.4kb homologous regions total). After selection on sucrose, I've found that 100% of my clones so far are WT and not the deleted mutant as desired. I'm wondering if ...

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