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Region

region

(Science: anatomy) A plane area with more or less definite boundaries.


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Why would the identical amino acids region of certain specie

OK, so you have DNA sequences from related species which are somewhere diverse, somewhere are identical. For example the region coding for active site will be probably conserved, while some loops on surface of the protein will be divergent. Both on DNA and amino acid level. You can ...

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by JackBean
Mon Feb 18, 2013 3:51 pm
 
Forum: Genetics
Topic: Why would the identical amino acids region of certain specie
Replies: 2
Views: 179

Why would the identical amino acids region of certain specie

... amino acids are identical, the DNA sequences are probably also very similar (but does not have to be identical!), thus primer annealing to this region may be used for several species. It can be used either as unspecific primer for known species or it can be used even for so far unsequenced species.

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by JackBean
Mon Feb 18, 2013 1:06 pm
 
Forum: Genetics
Topic: Why would the identical amino acids region of certain specie
Replies: 2
Views: 179

Why would the identical amino acids region of certain specie

Why would the identical amino acids region of certain species be used as a basis for a primer design? I know they share homology and are very conserved but why is it used as a primer?

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by TToe
Mon Feb 18, 2013 10:37 am
 
Forum: Genetics
Topic: Why would the identical amino acids region of certain specie
Replies: 2
Views: 179

Re: PCR

... band mass variation. When you are using 12 pairs of primers, they are targeting 24 different binding loci. Each forward and reverse pair spans a region that has a variable number of base pairs between individuals, but a given individual will have a definite base distance between each pair of ...

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by jonmoulton
Tue Jan 29, 2013 4:16 pm
 
Forum: Genetics
Topic: PCR
Replies: 2
Views: 502

PCR

I realise that each of the 12 primer pairs bind to a different region of human genome; the VNTR's within each region, and that is how 12 distinct bands can be detected after PCR. But what i don't know is why the exact same primers that target the same location ...

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by Julian92
Tue Jan 29, 2013 3:10 pm
 
Forum: Genetics
Topic: PCR
Replies: 2
Views: 502
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