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Reducing enzyme

reducing enzyme --> reductase

(Science: enzyme) An enzyme that catalyses a reduction; since all enzymes catalyze reactions in either direction, any reductase can, under the proper conditions, behave as an oxidase and vice versa, hence the term oxidoreductase. For individual reductase's, see the specific names.

Synonym: reducing enzyme.

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restriction reaction on agarose gel slices

I'm thinking of reducing steps for insert production. ATM I have following steps: 1. PCR 2. agarose gel with PCR products 3. gel extraction of insert of interest 4. restriction reaction 5. enzyme removal <- I often use KpnI/BamHI and can't go with heat inactivation 6. ligation ...

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by dustman
Fri May 25, 2012 2:54 pm
Forum: Molecular Biology
Topic: restriction reaction on agarose gel slices
Replies: 2
Views: 1803

Reasons for Skepticism on the Endosymbiosis?

... et al., 1994 ). In the case of linear chromosomes, the DNA polymerase enzymes are unable to replicate right to the end of the chromosome. This ... at a time extremely early in the history of eukaryotes, substantially reducing the window of time in which gene transfer could have occurred. Summary ...

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by SuperfluousAvatar
Thu Jan 12, 2012 7:43 pm
Forum: Cell Biology
Topic: Reasons for Skepticism on the Endosymbiosis?
Replies: 0
Views: 7603

HCL instead of imidazole!

... pH 3, third with pH 4...). Does your native protein turn yellow in the reducing buffer. I don't see much, how is that relevant. I meant, whether are you able to measure activity of your enzyme or is it some protein, which does not have catalytic activity (at least not ...

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by JackBean
Fri Dec 16, 2011 11:57 am
Forum: General Discussion
Topic: HCL instead of imidazole!
Replies: 6
Views: 2536

Irrefutable Facts Against Evolution

... magnetic field. Scientists have found that the magnetic field is reducing in strength at a rapid rate, with a half life of about 1,400 years. ... which evolution is quite contradictory to. 5)Lysosome Lysosome contain enzymes that are used to break down material and get it ready for disposal. ...

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by Tomn
Tue Oct 11, 2011 12:49 am
Forum: Evolution
Topic: Irrefutable Facts Against Evolution
Replies: 52
Views: 77886

Bio questions, help please

... explain explain (refering to amino acids) 6. Why is sucrose a non-reducing disaccharide? explain explain 7. would you expect the concentration ... able to move in and out of cells by way of diffusion? 17. Explain how enzymes function to speed up chemical reactions? explain explain explain ...

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by Rinatachka
Tue Jul 27, 2010 3:58 pm
Forum: General Discussion
Topic: Bio questions, help please
Replies: 3
Views: 5974
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