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Recombinant dna

Recombinant dna

(Science: molecular biology) Spliced dNA formed from two or more different sources that have been cleaved by restriction enzymes and joined by ligases. Genetically engineered dna made by recombining fragments of dna from different organisms.The joining together of genetic material from two different organisms.


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Re: Recombinant DNA not degraded inside transformed cells, why?

I found some explanation here: (last paragraph) http://www.tru.ca/faculty/dnelson/courses/biol335/335notes/3recdna/1-restriction/recDNA1.html Thanks.

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by Holmes
Wed Oct 03, 2012 7:25 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 1380

Re: Recombinant DNA not degraded inside transformed cells, why?

Here's one from PLOS Genetics today, right on time. Zhang G, Wang W, Deng A, Sun Z, Zhang Y, et al. (2012) A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria. PLoS Genet 8(9): e1002987. doi:10.1371/journal.pgen.1002987 http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1002987

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by jonmoulton
Fri Sep 28, 2012 6:18 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 1380

Re: Recombinant DNA not degraded inside transformed cells, why?

Escape from restriction endonucleases is a valid point. That is totally a chance dependent phenomenon. Thanks.

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by Holmes
Fri Sep 28, 2012 2:32 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 1380

Recombinant DNA not degraded inside transformed cells, why?

Why is it that recombinant DNA (a foreign DNA) not degraded inside the transformed bacteria? All bacterial systems have a system to distinguish ...

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by Holmes
Thu Sep 27, 2012 7:38 am
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 1380

Re: Compare and contrast the processes of Mitosis and Meiosis

... first division is reductional while the second division is equational. DNA is recombinant in daughter cell and not same as that of parent cell unlike mitosis. If u need ...

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by Satyaki
Thu Sep 06, 2012 3:44 am
 
Forum: Cell Biology
Topic: Compare and contrast the processes of Mitosis and Meiosis
Replies: 3
Views: 8275
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