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Recombinant dna

Recombinant dna

(Science: molecular biology) Spliced dNA formed from two or more different sources that have been cleaved by restriction enzymes and joined by ligases. Genetically engineered dna made by recombining fragments of dna from different organisms.The joining together of genetic material from two different organisms.


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Re: Gene targeting

... major's year sequence) microbiology cell biology molecular biology recombinant DNA techniques microbial genetics molecular genetics plant physiology evolution instrumental ...

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by jonmoulton
Thu Apr 25, 2013 6:11 pm
 
Forum: General Discussion
Topic: Gene targeting
Replies: 17
Views: 7785

Re: Recombinant DNA not degraded inside transformed cells, why?

I found some explanation here: (last paragraph) http://www.tru.ca/faculty/dnelson/courses/biol335/335notes/3recdna/1-restriction/recDNA1.html Thanks.

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by Holmes
Wed Oct 03, 2012 7:25 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 4699

Re: Recombinant DNA not degraded inside transformed cells, why?

Here's one from PLOS Genetics today, right on time. Zhang G, Wang W, Deng A, Sun Z, Zhang Y, et al. (2012) A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria. PLoS Genet 8(9): e1002987. doi:10.1371/journal.pgen.1002987 http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1002987

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by jonmoulton
Fri Sep 28, 2012 6:18 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 4699

Re: Recombinant DNA not degraded inside transformed cells, why?

Escape from restriction endonucleases is a valid point. That is totally a chance dependent phenomenon. Thanks.

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by Holmes
Fri Sep 28, 2012 2:32 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 4699

Recombinant DNA not degraded inside transformed cells, why?

Why is it that recombinant DNA (a foreign DNA) not degraded inside the transformed bacteria? All bacterial systems have a system to distinguish ...

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by Holmes
Thu Sep 27, 2012 7:38 am
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 4699
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