Recombinant

... sugars don't play a crucial role, but here you might want them in their right places). Then again, if I recall correctly, they make (humanised) recombinant proteins for therapeutical uses even in E. coli (a species that has no clue about glycosylation whatsoever...) Another problem you may encounter ...

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Looking for advice on my current situation. I have confirmed the recombinant protein I'm working to collect is expressed in T7 ecoli/pET24a. And after addition of lysozyme and then sonication the supernatant also showed the expected protein. INFO: the protein's ...

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... to get some basic info on science related terms. Thus for the G protein: http://en.wikipedia.org/wiki/G_protein#Heterotrimeric_G_proteins the recombinant His-tagged proteins is a under this: http://en.wikipedia.org/wiki/Polyhistidine-tag Hope this helps you to get started.

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... done some research on how PCR (polymerase chain reaction) works. However, I still do not understand the following things: - Protein G genes - Recombinant His-tagged proteins (and how they are purified by affinity chromatography on a nickel-agarose column) Thank you very much for your help! ...

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... relatively easy to engineer: in research and medicine bacterial genome is often manipulated in order to make them produce some desired protein. Recombinant human insulin is a very good example of a drug that is produced in bacteria. Humulin N is the trade name of one such insulin product. Try ...

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