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Recognition sequence

recognition sequence

A nucleotide sequence --typically composed of 4, 6, or 8nucleotides -- that is recognised by a restriction endonuclease. Type II enzymes cut (and theircorresponding modification enzymes methylate) within or very near the recognition sequence.


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Find number of restriction fragment size in chromosome

... in entrance exam. I need help. A restriction endonuclease has the recognition sequence G/AATTC, where "/" indicate the cut side. This sequence is found, on average, ...

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by kanagasundar
Wed Nov 21, 2012 1:38 am
 
Forum: Molecular Biology
Topic: Find number of restriction fragment size in chromosome
Replies: 1
Views: 1074

λ Phase Genetic Switch

... inserts his DNA by recombination; the integration is site-specific, the recognition-site is found to by 15bp long and is located between the gacactose ... My thesis: as long as the plasmid DNA doesn`t contain the recognition-sequence, the integration of the λ-DNA is restricted to the bacterial ...

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by lenni
Thu Nov 01, 2012 10:08 am
 
Forum: Microbiology
Topic: λ Phase Genetic Switch
Replies: 1
Views: 1334

Camouflage in nature

... a set of simple light sensitive cells which become useful for shades recognition, then a cup develops around them which enhances the process, ... does serve the purpose even in its simplicity . So the following sequence of mutations in Dawkins’ example only enhanced something that was ...

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by Nick7
Fri Nov 11, 2011 12:02 am
 
Forum: Evolution
Topic: Camouflage in nature
Replies: 16
Views: 12675

Plz help - question on probabilty of a nucleotide sequence

... restriction sites likely to be present in this DNA molecule? (a) BamHl (recognition sequence = GGATCC) My attempt at solving it: (.19) chance of getting an A or T (.31) chance ...

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by james0222
Fri Mar 25, 2011 3:53 am
 
Forum: Genetics
Topic: Plz help - question on probabilty of a nucleotide sequence
Replies: 4
Views: 3212

Re: Determining mRNA sequences

... is about nonsense mediated decay and how the pre-termination codon recognition is mediated by a competition between 3' UTR associated factors ... NMD. A mechanism of recruiting these factors could be through sequence-specific binding to a sequence element in the 3' UTR. By using the ...

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by id10t
Thu Mar 03, 2011 8:04 am
 
Forum: Molecular Biology
Topic: Determining mRNA sequences
Replies: 2
Views: 2068
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