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Reannealing

reannealing

renaturation of a dNA sample that has been dissociated by heating. In reannealing the two strands that recombine to form a double stranded molecule are from the same source. Differences in the rate of reannealing led to the early recognition of repetitive sequences which rapidly recombine (have low C0t values).


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PLease Please I need help with my PCR! Greatly appreciated!

I want to know the RE you have used in PCR. The patterns are correct as it seems. I think there is some problem in RE along with Denaturation and Reannealing. Procedure is correct as it seems. In your final conclusion, you have mentioned about the 585bp of lane 5 or 7? I am little uncleared about ...

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by greatmicrobiologist
Tue Aug 30, 2011 7:29 am
 
Forum: Molecular Biology
Topic: PLease Please I need help with my PCR! Greatly appreciated!
Replies: 8
Views: 7414

DNA Gyrase vs. DNA Helicase

... hydrogen bonds of the base pairs of nucleotides. DNA gyrase relieves the tension from the unwinding of the double helix by cutting the strand, and reannealing it once the tension has been released.

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by Jennifer
Wed Nov 30, 2005 1:37 am
 
Forum: Molecular Biology
Topic: DNA Gyrase vs. DNA Helicase
Replies: 4
Views: 6882


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