Reagents

... much more reliably than a lone molecular biologist would do, since they have established protocols for quality control, standardized equipment and reagents, and long tradition in making these tests. They have a degree of inaccuracy like all scientific methods do, but unless you are a competent ...

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I have a question about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These ...

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... in protocol procedure, sometime do not understand the way they write their experimental data. For instance, a Buffer that consists of a multible reagents and labeled (10X). I was asked to dilute it by taking 3 ml of that 10x buffer and add 30 ml of distelled water and I was told that this will ...

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... tubes, tips, pipets, and such for contamination. Obtain (make/borrow/buy) new controls and run them along with the old ones. Use all new reagents in case it was pipet contamination and your “new” reagents are already contaminated…

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... cycler conditions and PCR rxn mix followed factory recommended protocols. In all cases the primers, enzymes (Advantage 2 from Clontech) and other reagents were tested and they work fine with controls. My question: to me everything points to the quality of the cDNA, maybe low concentration of the ...

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