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Reagents

reagent strips

narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances.


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amplification in no RT control

... later than RT+ samples. I am getting single peak melt curves and my NTC is not amplifying. This suggests to me that I am not getting dimers and my reagents are 'good' I have tried cDNA at 10ng and 5ng and primer conc. at both 200uM and 400uM. I'm kind of out of ideas now...more/longer DNase digestion? ...

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by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 1
Views: 64

Methylene Blue Solution use in staining variety of bacteria?

... that can be an endotoxin (toxic shock syndrome). Gram stain experiments are another type of a multi-step staining and de-staining experiment. Reagents 0.1M Sodium Acetate Buffer, pH4.2: Sodium acetate, trihydrate (MW 136.1) ------ 1.36 g Distilled water ----------------------------------- ...

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by scytek
Sun Feb 22, 2015 9:22 am
 
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 275

Re: An interesting PCR smearing...I dont know what to do :(

... amplification is not coming properly.and this has occured recently. ( even though the PCR reaction was standardized earlier ) I have used same reagents , pipettes , PCR machine , reconstituted new primers for amplifying all 3 exons . Interestingly sometimes I do get a single bright band , but ...

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 8781

Propagation and Transfection of BHK cells?

... reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high transfection efficiency in hard to transfect cells like kidney fibroblasts as well as in standard cells, you can work with complete ...

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by OliviaZ
Tue Apr 08, 2014 10:48 am
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 2636

Overexpression

... reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high transfection efficiency in hard to transfect cells like Primary macrophages as well as in standard cells, you can work with complete ...

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by OliviaZ
Tue Apr 08, 2014 10:45 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 2978
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