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reagent --> periodic acid Schiff reaction

(Science: technique) A histochemical technique based on periodic acid oxidation of a substance containing the 1,2-glycol grouping.

It is used for staining carbohydrates as the resulting dialdehyde reacts with Schiff reagent to form a coloured product.

The adjacent hydroxyl groups are oxidized to form aldehydes by periodic acid (HIO4) and these aldehyde groups react with Schiff's reagent (basic fuchsin decolourised by sulphurous acid) to give a purple colour.

Acronym: PAS

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it doesn't have to be only the buffer but any additive you're using other additive that I used is antimicrobial reagent-tetracycline HCL which is I used for prevent any contamination in the reaction but I think there is no difference after added this reagent, means that OD blank ...

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by intandiana
Sun Jan 19, 2014 5:59 am
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1161

Simulated intracellular fluid

It is difficult to simulate intracellular fluid by using chemical reagent. Another way is using NP40 or OG to lysate the cell and collect the cell lysate. Then spin down the proteins and get the intracellular fluid. Will ___________________________________________________________________________________________ ...

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by agb06
Fri Sep 13, 2013 2:28 am
Forum: Cell Biology
Topic: Simulated intracellular fluid
Replies: 1
Views: 749

neuron staining - Golgi cox reagent

... graduate, gathering all the informatio i require before setting out for a project;on 'the evolution of neurons of chickens' but the Golgi cox reagent seem to be the problem. Please any one who has an idea of the components and the compositions for proper staining and the standard procedure,and ...

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by fitzkefas
Sun Sep 08, 2013 6:27 pm
Forum: Genetics
Topic: neuron staining - Golgi cox reagent
Replies: 0
Views: 582

indole test reaction

... be used for such biochemical test ( it has Typtose), i incubated the 5 tubes (with inoculum) at 44.5 degrees and after 24 hrs. i dropped kovacs reagent to the tubes, after a while 1 tube has a red ring while the other tubes has green ring. And then after a while the tube with the red ring suddenly ...

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by magandangbutiki
Fri Aug 02, 2013 7:53 am
Forum: Microbiology
Topic: indole test reaction
Replies: 0
Views: 463

Protein isolation

... not grown as 2D monolayers (I am looking at cell aggregates to be specific). Our original plan is to isolate the protein using trizol extraction reagent, then run an ELISA on HIF 1 alpha. However, I am worried that the protocol may not work. I haven't heard of trizol being used for a specific ...

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by roth317
Tue Feb 12, 2013 9:25 pm
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 1580
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