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Reading frame open

reading frame, open

An open reading frame in dNA has no termination codon, no signal to stop reading the nucleotide sequence, and so may be translated into protein.

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Sequencing question/primer design

... primer for XXX gene sequence that is in frame (in frame as in the Open reading frame w/ ATG?) and removes the signal peptide. Additionally, ...

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by coppcw08
Sun Nov 04, 2012 8:19 pm
Forum: Molecular Biology
Topic: Sequencing question/primer design
Replies: 4
Views: 3847

Can I deduce the Number of Proteins Produced?

I have a question given to me, reading: Calculate the number of proteins with an ... genome assuming that 80% of the a genome contain open reading frames. The average molecular weight of an amino acid is 50 ...

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by blah484
Thu Jun 28, 2012 3:53 pm
Forum: Genetics
Topic: Can I deduce the Number of Proteins Produced?
Replies: 1
Views: 2512

what is six-frames translation

... ?: "We know that there are six possible reading frames for any given nucleotide sequence. Generally the longests Open reading frame is taken to be the correct frame, when we do ...

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by genetherapy
Mon Mar 19, 2012 12:47 pm
Forum: Molecular Biology
Topic: what is six-frames translation
Replies: 4
Views: 8846

Re: SiHa cells cloning

... transgene contains Green fluorescent protein open reading frame, which is expressed, but it isn't toxic for SiHa cells. I believe ...

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by jappy
Sat Sep 26, 2009 6:48 pm
Forum: Cell Biology
Topic: SiHa cells cloning
Replies: 8
Views: 4507

Two questions about operon (transcription/translation)

... have a ribosome binding site (RBS) for each open reading frame. However, others do not: sometimes the last codon of one open ...

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by MrMistery
Sun Aug 02, 2009 11:27 pm
Forum: General Discussion
Topic: Two questions about operon (transcription/translation)
Replies: 2
Views: 8360
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