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Reaction

reaction

standard method for identifying clostridium perfringens. When grown on agar containg egg yolk, an opalescent halo is formed around colonies that produce _ toxin (lecithinase).


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Gateway technology

... and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression clone of a certain gene? Why can't I use an attL-PCR product or a linearized attL DNA and make a reaction ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 71

Re: An interesting PCR smearing...I dont know what to do :(

... well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured recently. ( even though the PCR reaction was standardized earlier ) I have used same reagents , pipettes , PCR machine , reconstituted new primers for amplifying all 3 exons . Interestingly ...

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 6271

Evolutionary psychology

... to some people in better understanding emotion. Instinct is "an inherited tendency of an organism to behave in a certain way, usually in reaction to its environment and for the purpose of fulfilling a specific need..." American Heritage Science Dictionary 1) "inherited"- ...

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by 1300
Mon Apr 28, 2014 12:50 am
 
Forum: Evolution
Topic: Evolutionary psychology
Replies: 0
Views: 508

Re: Transpiration and Photosynthesis.

Try to consider the light and dark reaction. A plant it active during the day and photosynthsise and in the process release excess H2O contents to the atmosphere (transpiration). So the phenomenom are complimentary.

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by perfection
Mon Apr 07, 2014 7:55 am
 
Forum: Botany Discussion
Topic: Transpiration and Photosynthesis.
Replies: 4
Views: 2480

Help: Confused with primers in a PCR of a vector?

... E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This PCR reaction is something I do not really understand. We used the pBS-gene as our template. We carried out ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2218
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