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Re: In Vitro Transcription Translation

... (location of gel band). However, if you need to unambiguously prove the identity of the protein you might need a Western as well, since your radiolabelled gel gives mass information but not an epitope-antigen interaction. If you have a single mRNA produced in the transcription-translation ...

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by jonmoulton
Wed Jun 05, 2013 10:59 pm
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 2054

Radio-phosphate binding assay

... in phosphate solution for 1 hour. 5) Develop blot in phospho-imaging casette. Leave overnight and observe. The phosphate solutio contains 5 uCi radiolabelled P-32 (beta emission), suspended in 10ml 10mM Tris-Cl buffer ph 7.0. I expect to see something on my developed phosphorescent plates, but ...

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by vader1941
Tue Jul 10, 2012 3:32 pm
Forum: Molecular Biology
Topic: Radio-phosphate binding assay
Replies: 0
Views: 1578

Re: Deproteinizing plasma?

Yes, blood plasma. I need to infuse radiolabelled glucose in vivo and measure hepatic glucose production. I am just not sure the best way to deproteinize the blood to get the infused glucose vs. blood glucose values?

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by ntb103
Tue Jan 19, 2010 2:15 pm
Forum: Physiology
Topic: Deproteinizing plasma?
Replies: 10
Views: 9461

sanger sequencing

... of one or two of the four nucleotide bases in each of four reactions (G, A+G, C, C+T). Thus a series of labelled fragments is generated, from the radiolabelled end to the first 'cut' site in each molecule. The fragments are then size-separated in a gel, with the four reactions arranged side by ...

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by CyLMH
Mon Mar 19, 2007 1:50 pm
Forum: Molecular Biology
Topic: sanger sequencing
Replies: 2
Views: 3396

Positron emission Tomography

... fMRI scanners are capable of detecting areas of molecular biology detail (even prior to anatomic change). The PET scanner does this via the use of radiolabelled molecular probes that have different rates of uptake, depending on the type and function of tissue involved. The changing of regional ...

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by dipjyoti
Wed Sep 27, 2006 9:57 am
Forum: Human Biology
Topic: Positron emission Tomography
Replies: 0
Views: 3505

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