Radioactivity

... 1ul (15U) of TdT The reaction mix was incubated at 37C and after 5min, 2ul aliquots were removed from the reaction mix to determine the amount of radioactivity incorporated. One aliquot was counted directly to determine the total amount of radioactivity in the sample. Another aliquot was TCA precipitated ...

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... into a nucleotide chain, and that might also present problems on tRNA loading. OK, so it's not a great idea, but if you don't want to radioactivity its the only thing I can think of

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Radioactive amino acids are introduced into a cell.In which cell structure will the radioactivity first become concentrated?

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... solution of a DNA fragment labelled with 32P with an initial activity of 0.4 kBq in 10 ml was needed for an experiment 9 days later. Calculate the radioactivity of the sample used in the experiment (T = half-life) (I think I first need to work out the amount of radioactivity in the sample to be ...

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... the tracer to the amount of dpm's you need. The contribution of tracer to the total concentration of substrate is negligible. You would use the radioactivity as a monitor of %conversion which will range from 0-100%. But if you are going to isolate the product and look at the distribution of ...

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