Dictionary » R » Radial immunodiffusion

Radial immunodiffusion



A quantitative immunodiffusion technique used to detect the level of protein (antigen) in a sample by measuring the diameter of the ring of precipitin formed by the complex of the protein (antigen) and the antiserum (antibody).


The procedure involves placing of a solution of protein (antigen) into a well cut into an agarose gel containing the evenly distributed antiserum (antibody). As the protein (antigen) diffuses out of the well, it complexes with the antiserum (antibody) and forms a precipitin ring, the diameter of which is proportional to the quantity of the protein (antigen) in the well.

Compared with microbial diffusion assays, this technique is less precise as it gives a rather relatively insensitive estimation of the concentration of antigen. However, it has the advantage of specificity. Thus, it can be applied when sensitivity is not required but specificity is, and antibody is plentiful. It is commonly used in clinically detecting levels of blood proteins in a patient.

See also: antigen.

Please contribute to this project, if you have more information about this term feel free to edit this page

Results from our forum

Analyzing lysozyme in biological fluids

Other papers I have looked at use radial immunodiffusion. Take a look at this: http://www.bindingsite.co.uk/uk/media/file/MKG3414E.pdf. Look at ...

See entire post
by aptitude
Sun Sep 11, 2011 8:30 pm
Forum: Molecular Biology
Topic: Analyzing lysozyme in biological fluids
Replies: 1
Views: 1632

This page was last modified 08:01, 31 July 2008. This page has been accessed 17,897 times. 
What links here | Related changes | Permanent link