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R plasmids

(noun) Plural form of R plasmid.

See R plasmid for definition and additional information.


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Propagation and Transfection of BHK cells?

... If you are interested I may send a free sample to you. We provide three transfection reagents: for siRNA (Viromer BLUE, BLACK and GREEN) and for plasmids (Viromer RED and YELLOW). Please write to: Olivia.Zabel@lipocalyx.de (email address of our company). Best wishes, Olivia

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by OliviaZ
Tue Apr 08, 2014 10:48 am
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 1152

Overexpression

... in buffer. If you are interested I may send a free sample to you. We provide two transfection reagents: for siRNA (Viromer BLUE and GREEN) and for plasmids (Viromer RED and YELLOW). Please write to: Olivia.Zabel@lipocalyx.de (email address of our company). Best wishes, Olivia

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by OliviaZ
Tue Apr 08, 2014 10:45 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1445

plasmid extraction on 1.5% agarose gel

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates

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by cah2010
Wed Dec 18, 2013 2:23 am
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 1564

How

... need a bit more details of what you want to do. But technically if you just want to have 2genes one after the next to insert you could either plasmids with multiple insertion sites, make your construction in those (easy) then excise the double insert and transfer it to the genomic DNA as you ...

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by canalon
Sat Nov 02, 2013 6:15 pm
 
Forum: Molecular Biology
Topic: How
Replies: 4
Views: 1532

Overexpression

Sorry, I have no experience with cell cultures as yours, but from what I can say: Your location doesn't really matter for ordering plasmids, you will probably buy it from some big company anyway. Just find the best vector for you (including selection). GFP is IMHO suitable for single cell ...

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by JackBean
Thu Sep 05, 2013 11:41 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1445
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