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R plasmids

(noun) Plural form of R plasmid.

See R plasmid for definition and additional information.


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plasmid extraction on 1.5% agarose gel

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates

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by cah2010
Wed Dec 18, 2013 2:23 am
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 815

How

... need a bit more details of what you want to do. But technically if you just want to have 2genes one after the next to insert you could either plasmids with multiple insertion sites, make your construction in those (easy) then excise the double insert and transfer it to the genomic DNA as you ...

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by canalon
Sat Nov 02, 2013 6:15 pm
 
Forum: Molecular Biology
Topic: How
Replies: 4
Views: 903

Overexpression

Sorry, I have no experience with cell cultures as yours, but from what I can say: Your location doesn't really matter for ordering plasmids, you will probably buy it from some big company anyway. Just find the best vector for you (including selection). GFP is IMHO suitable for single cell ...

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by JackBean
Thu Sep 05, 2013 11:41 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 1
Views: 668

MOLECULAR CLONING

... taken up by competent cells and colonies will grow. Any comment on this? Will those nick be gone and will bacteria amplify intact circular dsDNA plasmids? tx

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 4104

HIS3-MX6 selective marker in S.cerevisiae

... yeast transformations. I was wondering if anybody had similar problems and would have a solution (like a specific way to transform yeasts with plasmids containing HIS3-MX). I try to transform an integrating PCR fragment that contains HIS3-MX6 marker but I don't recover any transformants on ...

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by cathbr
Fri Jul 05, 2013 6:51 pm
 
Forum: Molecular Biology
Topic: HIS3-MX6 selective marker in S.cerevisiae
Replies: 0
Views: 464
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