R plasmid

... only thinkable explanation involving a contamination would be if the extraction of the cellular DNA went wrong. Do you have the gene already in a plasmid anywhere in your lab? We once found out that we had plasmid contamination in autoclaved water. When you use this water for PCR, you get a band... ...

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... just incase the genes should spit, which there is a chance of if you're trying to splice the gene into a larger sequence or if it is just a plasmid. because then you might have reporters getting made without the protein of interest (false reports) or proteins of interest that are not 'reported'. ...

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... as some of the MHC genes that dictate tissue antigenicity. The construct that is transfered into the pig genome can be multiplied in a bacterial plasmid. Afaik, the modified cells themselves are rarely grown in a culture for somatic cell transfer (which is, in effect, cloning). The standard method, ...

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... DNA. 2. To genetically modify a pig, a DNA construct (artificially made string of nucleotides, which have genetic information) and a bacterial plasmid are added into a test tube. 3. I have no idea how they create modified cells or whatever... 4. The modified cells are grown in a culture? 5. ...

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... 3.1. Then I ligated the deletion constructs in 4 seperate reactions with the vector pCDNA, picked the transformation colonies, grew, extracted the plasmid, and digested a small amount of the plasmid with the same enzymes to make sure there was correct insert. After this visual confirmation, I transfected ...

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