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R plasmid

Definition

noun, plural: R plasmids

Shortened term for resistance plasmid that confers certain bacterial strain the acquired ability to resist the antimicrobial activity of some drugs.


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Please guide me about these restriction enzymes

you must select enzyme that cut your plasmid in one (or two) determined sequense and also not your target gene.

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by hematologym
Mon Mar 17, 2014 11:17 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 173

Help: Confused with primers in a PCR of a vector?

... a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This PCR reaction is something I do not really understand. We used the pBS-gene ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 0
Views: 65

Please guide me about these restriction enzymes

I guess that depends rather on your plasmid https://www.neb.com/products/r0189-noti https://www.neb.com/products/r0131-nhei

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by JackBean
Tue Mar 11, 2014 2:20 pm
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 173

Please guide me about these restriction enzymes

For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI? please compare and describe them

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by 2angel
Tue Mar 11, 2014 7:24 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 173

plasmid extraction on 1.5% agarose gel

http://www.bioinformatics.nl/molbi/SimpleCloningLab/electrophoresis.htm check the part about plasmid DNA

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by JackBean
Thu Dec 26, 2013 5:56 pm
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 815
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