RNase

you can find out what RNase is by googling it. And really, you should try to understand the basic processes in biology before attempting to answer difficult questions.

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Can someone also explain what blcr11 means (i.e. what RNAse is and what it does, etc.)?

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... of CsCl, will band the plasmid in one place, and the chromosomal DNA in another place along the gradient. The RNA should have been digested by RNAse as well as by the alkaline treatment and should pellet at the bottom of the gradient, safely out of the way. When you carefully remove the bands, ...

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"Knockdown" implies an oligo-based method. This could be actually introducing an oligo into cells, such as one of the following: RNase-H competent antisense -- ssDNA oligos ssRNA oligos Phosphorothioate oligos Many sorts of chimeric oligos RNase-independent antisense -- Morpholino ...

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... You need to run a special gel for mRNA. It needs to be a denaturing (formaldehyde) gel and it needs to be performed in a gel box that is RNase-free, with RNase-free buffer, etc. Before being loaded onto the gel, your sample needs to be heated in formaldehyde to denature it. If you don't ...

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