RNA polymerase

Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the explanation to that. ...

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... and shape) 3) protein-protein interactions (again, affected also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors Thanks a lot

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... and shape) 3) protein-protein interactions (again, affected also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors

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... etc). In 2006, Alleman et al proposed that "paramutation is RNA-directed. Stability of the chromatin states associated with paramutation ... silencing requires the mop1 gene, which encodes an RNA-dependent RNA polymerase." This polymerase is required to maintain a threshold level ...

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... is mainly that bases can be lost at the ends, or that it can ligate and produce a molecule that is missing a few bases, which get filled in by the polymerase of the bacterium after you transform your ligation mix. Oftentimes i'll get a whole bunch of re-ligated vector when I pick my colonies and ...

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