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RNA polymerase

Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from a dna template, a process termed transcription. In scientific terms, RNAP is a nucleotidyl transferase that polymerizes ribonucleotides at the 3' end of an rna transcript. Rna polymerase enzymes are essential and are found in all organisms, cells, and many viruses.


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The question about TATA sequence flexibility

... genes in archaea and eukaryotes that is responsible for recognizing the RNA-polymerase. http://en.wikipedia.org/wiki/TATA_box I read this article about TATA-Binding ...

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by Fantomas
Sat Aug 31, 2013 10:57 am
 
Forum: Molecular Biology
Topic: The question about TATA sequence flexibility
Replies: 1
Views: 1693

RNA

Ques1: Phosphorylation of RNA pol which are the roles of the phosphorylaton of C terminal Domain of RNA polymerase? (more than one may apply) 1. It increases the speed of transcription 2. It increase the specificity ...

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by 2468
Tue Nov 20, 2012 6:03 pm
 
Forum: Molecular Biology
Topic: RNA
Replies: 6
Views: 15680

Questions on the Sodium Potassium Pump and Enzymes

... unwinding the double helix or when an enzyme has two jobs such as DNA Polymerase forming phosphodiester bonds between nucleotides and adding new nucleotides to the RNA Primer. Also, what is Proeolytic Cleavage?

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by JoWillyTso
Mon Nov 05, 2012 5:50 pm
 
Forum: Cell Biology
Topic: Questions on the Sodium Potassium Pump and Enzymes
Replies: 3
Views: 3673

Question regarding about PCR - DNA or RNA as template

... His questions is following - why DNA must be used for PCR and not RNA??? The question arised from following - I did an experiment which isolated ... cause 1. RNA will degrade during 95 C denaturation step or 2. DNA polymerase used for PCR is very specific for DNA. Do you guys have any other ...

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by Xenotoxic
Fri Aug 17, 2012 3:55 am
 
Forum: Molecular Biology
Topic: Question regarding about PCR - DNA or RNA as template
Replies: 1
Views: 3690

A question I'm finding hard to answer (recombinant protein)

The RNA Polymerase is different.

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by adobe
Thu Jul 12, 2012 8:36 am
 
Forum: Cell Biology
Topic: A question I'm finding hard to answer (recombinant protein)
Replies: 9
Views: 6765
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