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RNA polymerase

Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from a dna template, a process termed transcription. In scientific terms, RNAP is a nucleotidyl transferase that polymerizes ribonucleotides at the 3' end of an rna transcript. Rna polymerase enzymes are essential and are found in all organisms, cells, and many viruses.


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Re: PCR & Agarose gel electrophoresis

Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the explanation to that. ...

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by apolll
Sun Aug 23, 2009 8:40 pm
 
Forum: Molecular Biology
Topic: PCR & Agarose gel electrophoresis
Replies: 3
Views: 120

influence of temperature on biomolecules

... and shape) 3) protein-protein interactions (again, affected also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors Thanks a lot

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by biobynight
Thu Aug 20, 2009 8:37 pm
 
Forum: Molecular Biology
Topic: influence of temperature on biomolecules
Replies: 0
Views: 62

Cellular processes affected by temperature..

... and shape) 3) protein-protein interactions (again, affected also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors

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by biobynight
Thu Aug 20, 2009 8:34 pm
 
Forum: Cell Biology
Topic: LIfe processes affected by temperature..
Replies: 3
Views: 589

Paramutation

... etc). In 2006, Alleman et al proposed that "paramutation is RNA-directed. Stability of the chromatin states associated with paramutation ... silencing requires the mop1 gene, which encodes an RNA-dependent RNA polymerase." This polymerase is required to maintain a threshold level ...

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by koan
Thu Aug 20, 2009 8:10 pm
 
Forum: Molecular Biology
Topic: Paramutation
Replies: 5
Views: 105

Re: Ligation and expression trouble

... is mainly that bases can be lost at the ends, or that it can ligate and produce a molecule that is missing a few bases, which get filled in by the polymerase of the bacterium after you transform your ligation mix. Oftentimes i'll get a whole bunch of re-ligated vector when I pick my colonies and ...

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by MrMistery
Thu Jul 09, 2009 12:41 am
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396
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