Dictionary » R » RNA polymerase

RNA polymerase

Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from a dna template, a process termed transcription. In scientific terms, RNAP is a nucleotidyl transferase that polymerizes ribonucleotides at the 3' end of an rna transcript. Rna polymerase enzymes are essential and are found in all organisms, cells, and many viruses.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Taqman probe design using FISH probe (need help)

... temperature such that it will remain hybridized at PCR extension temperature (but not so high as a fish probe) and will be hydrolysed by Taq DNA polymerase at every replication, thus releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of ...

See entire post
by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 574

Re: Purpose of a primase/polymerase localized to the cytosol?

Hi, I have some naïve thoughts about this situation: If it is an RNA pol, it may be helping maintain/regulate RNA levels in cytosol by e.g. recharging decaying RNA levels in cytosols for extended translation. If it is a DNA pol, it might be a long surviving ...

See entire post
by Hrushikesh
Fri Dec 12, 2014 8:40 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1576

Purpose of a primase/polymerase localized to the cytosol?

Hi, I recently read a paper, "PrimPol, an Archaic Primase/Polymerase Operating in Human Cells" by Garcia-Gomez et al. This polymerase was largely localized to the cytosol - I'm wondering if anyone has ideas as to why that would be the case or ...

See entire post
by rhardison
Fri Oct 03, 2014 8:51 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1576

Re: Any ideas for vaccine development or anti-viral drugs???

... effectively interfere with the replication of several positive-strand RNA viruses in cell culture. The filoviruses, Marburg virus and Ebola virus ... of viral mRNAs for the viral proteins (VPs) VP24, VP35, and RNA polymerase L protected rodents in both pre- and post-exposure therapeutic ...

See entire post
by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12665

Annealing Temparature

... at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

See entire post
by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 2601
View all matching forum results

This page was last modified 08:06, 22 February 2007. This page has been accessed 77,267 times. 
What links here | Related changes | Permanent link