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RNA-dependent DNA polymerase

Definition'

noun

A DNA polymerase enzyme that catalyzes the process of reverse transcription.


Supplement

This enzyme makes use of RNA molecule as a template for the synthesis of a complementary DNA strand. It is produced by HIV and other retroviruses in order for them to synthesize DNA from their viral RNA.

It is also found to be involved in the movement of certain mobile genetic elements, such as the Ty plasmid in yeast, in the replication of other viruses such as hepatitis B and possibly in the generation of mammalian pseudogenes.

It was discovered in 1970 by two independent scientists, Howard Temin and David Baltimore.

Synonym: reverse transcriptase, transcriptase.
See also: reverse transcription.


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Purpose of a primase/polymerase localized to the cytosol?

Hi, I recently read a paper, "PrimPol, an Archaic Primase/Polymerase Operating in Human Cells" by Garcia-Gomez et al. This polymerase was largely localized to the cytosol - I'm wondering if anyone has ideas as to why that would be the case or ...

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by rhardison
Fri Oct 03, 2014 8:51 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 0
Views: 23

Annealing Temparature

... at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
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Views: 1580

Annealing Temparature

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by JackBean
Tue Feb 18, 2014 12:40 pm
 
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Topic: Annealing Temparature
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A question regarding PCR

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by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
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Taq Buffer ?

why Taq DNA Polymerase Buffers containing Gelatin ?

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by Helics
Sat Jan 18, 2014 9:36 am
 
Forum: Molecular Biology
Topic: Taq Buffer ?
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