RES

Take a look at the DNA alignment of two genes: http://www.ebi.ac.uk/Tools/services/rest/emboss_needle/result/emboss_needle-I20121203-233912-0692-97003408-pg/aln The Homo Sapien is the longest DNA sequence. If the Homo Sapien gene has 1770 bases surely the overall aligned length should also be 1770, ...

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You should get complete library (if you use sufficient number of clones) with any REs. There is a calculation to get number of clones which you have to screen to get your gene of interest with some probability. The only thing which might be of concern could be telomeric ...

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Look at this web-sites,the informations that they have will help you http://www.molecularstation.com/dna/restriction-enzyme-digestion/ http://www.methodbook.net/dna/restrdig.html

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... music for lung tumor induction in mice. 2nd Int Conf on Qigong. Xian, China. 94E; 1989. 12. Hu, Zonglian; Chen, Huiying; Jiang, Shufang. Cancer Research Center, Nan-Jing Yang-Gong 81 Hospital, China [1]. Observation of Chinese qigong (Lu Ho Kuen outer qi) on inducing terminal granulocytic differentiation ...

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You have to find out, where your REs cut. Let's say it will be in positions 350, 1400 and 3050. Thus you will have fragments 1050 (1400-350), 1650 (3050-1400) and 600 (3300-3050+350). thanks for the reply but i didnt get anything, rather ...

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