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R17 bacteriophage

R17 bacteriophage

bacteriophage with rNA genome that codes for the enzyme RNA synthetase and for the coat protein, a protein to which the RNA is attached and that is involved in attachment to the bacterium.


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cellular mechanisms that result in symptoms of cholera

... the cholera toxin is introduced into V. cholerae by horizontal gene transfer. Virulent strains of V. cholerae carry a variant of temperate bacteriophage called CTXf or CTXφ.

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by adithya528
Tue Dec 11, 2012 1:49 pm
 
Forum: Cell Biology
Topic: cellular mechanisms that result in symptoms of cholera
Replies: 4
Views: 5238

Idea to determine order of assembly of spliceosome

... mail from my teacher and he actually suggested I do the precipitation, just as you did. From one thing to another though. What is the purpose of a bacteriophage promoter being ligated in to a vector along with your gene? Is it just to ensure that you get strong expression if you use some kind of ...

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by nukeman
Wed May 30, 2012 6:14 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7832

Idea to determine order of assembly of spliceosome

... My initial thought was to use in vitro splicing assay with a couple of extra steps. 1. Ligate a traget gene to a vector with some kind of bacteriophage RNA-pol promotor 2. In vitro transcription with radioactive nucleotides 3. Extraction of pre-mRNA 4. Run agarose-gel where I can identify ...

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by nukeman
Mon May 28, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7832

Re: Bacteriophages and Acne?

... Gram postive - so no classic endotoxin (LPS) tho' debris could still be a concern. But assuming topical application, this may be less of a worry. Bacteriophage therapy also suffers from host rimmune response to the phage itself, and those who have worked with bacteriophages will recall the typical ...

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by JorgeLobo
Sun Apr 01, 2012 8:59 pm
 
Forum: Microbiology
Topic: Bacteriophages and Acne?
Replies: 9
Views: 13050

Bacteriophages and Acne?

http://www.phagetherapycenter.com Bacteriophage Therapy for Patients Across the Globe!!! An Effective Treatment for Many Difficult Bacterial Infections Phage Therapy Center specializes in three particular situations where bacteriophage therapy ...

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by victory798
Sun Apr 01, 2012 8:42 pm
 
Forum: Microbiology
Topic: Bacteriophages and Acne?
Replies: 9
Views: 13050
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