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R17 bacteriophage

R17 bacteriophage

bacteriophage with rNA genome that codes for the enzyme RNA synthetase and for the coat protein, a protein to which the RNA is attached and that is involved in attachment to the bacterium.

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Radioactivity Q

... molecules. In an experiment to determine the rate at which the TdT added dNTPs to the 3’ end of DNA, the following experiment was set up: Linear-bacteriophage lambda DNA (48.5kb) at a concentration of 55ug ml^-1 was cut with the restriction enzyme Alu I. (Lambda DNA is cut 143 times with Alu ...

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by monkey07
Tue May 05, 2009 4:18 pm
Forum: Molecular Biology
Topic: Radioactivity Q
Replies: 0
Views: 386

plzz help me....

From the top of my head, i would suggest that you look up: - the use of bacteriophage to treat infectious diseases - use of viruses to transfer DNA in cells notably in gene therapy

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by canalon
Sun Mar 01, 2009 10:04 pm
Forum: General Discussion
Topic: plzz help me....
Replies: 3
Views: 525

Re: T4 phage strains

Try looking at the sections that describe how Benzer analyzed the fine structure of genes using bacteriophage. The parental strains will be the most common product--it doesn't require any recombination event to create the parentals. Since it is obvious from the data what ...

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by blcr11
Sat Jan 03, 2009 1:16 pm
Forum: Genetics
Topic: T4 phage strains
Replies: 2
Views: 561

Simple Protein Question

... or metabolic regulation. To be sure, most proteins in the cell do not contain phosphorus. But for those that do, it is important. The tagging of bacteriophage you mention sounds like a discussion of the Hershey-Chase experiment to prove that the transforming principle was DNA and not protein. ...

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by blcr11
Thu Dec 25, 2008 12:26 pm
Forum: Molecular Biology
Topic: Simple Protein Question
Replies: 2
Views: 489

Re: transfection

The original meaning of transfection was 'infection by transformation', i.e. introduction of DNA (or RNA) from an eukaryote virus or bacteriophage into cells, resulting in an infection. Because the term transformation had another sense in animal cell biology (a genetic change allowing long-term ...

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by sam2008
Thu Oct 16, 2008 6:56 am
Forum: Cell Biology
Topic: transfection
Replies: 2
Views: 671
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